New synchronization method for Plasmodium falciparum

Ranford-Cartwright, L.C., Sinha, A., Humphreys, G.S. and Mwangi, J.M. (2010) New synchronization method for Plasmodium falciparum. Malaria Journal, 9(170), (doi:10.1186/1475-2875-9-170)

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Abstract

Background: Plasmodium falciparum is usually asynchronous during in vitro culture. Although various synchronization methods are available, they are not able to narrow the range of ages of parasites. A newly developed method is described that allows synchronization of parasites to produce cultures with an age range as low as 30 minutes. Methods: Trophozoites and schizonts are enriched using Plasmion. The enriched late stage parasites are immobilized as a monolayer onto plastic Petri dishes using concanavalin A. Uninfected erythrocytes are placed onto the monolayer for a limited time period, during which time schizonts on the monolayer rupture and the released merozoites invade the fresh erythrocytes. The overlay is then taken off into a culture flask, resulting in a highly synchronized population of parasites. Results: Plasmion treatment results in a 10- to 13-fold enrichment of late stage parasites. The monolayer method results in highly synchronized cultures of parasites where invasion has occurred within a very limited time window, which can be as low as 30 minutes. The method is simple, requiring no specialized equipment and relatively cheap reagents. Conclusions: The new method for parasite synchronization results in highly synchronized populations of parasites, which will be useful for studies of the parasite asexual cell cycle.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Ranford-Cartwright, Dr Lisa and Mwangi, Dr Jonathan and Humphreys, Ms Georgina and Sinha, Dr Abhinav
Authors: Ranford-Cartwright, L.C., Sinha, A., Humphreys, G.S., and Mwangi, J.M.
Subjects:Q Science > QR Microbiology
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Malaria Journal
Publisher:BioMed Central
ISSN (Online):1475-2875
Published Online:17 June 2010
Copyright Holders:Copyright © 2010 The Authors
First Published:First published in Malaria Journal 9:170
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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