Yuan, Z.Q., Nicolson, L., Marchetti, B., Gault, E.A., Campo, M.S. and Nasir, L. (2008) Transcriptional changes induced by bovine papillomavirus type 1 in equine fibroblasts. Journal of Virology, 82(13), pp. 6481-6491. (doi: 10.1128/JVI.00429-08)
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Abstract
Bovine papillomavirus type 1 (BPV-1) and, less commonly, BPV-2 are associated with the pathogenesis of common equine skin tumors termed sarcoids. In an attempt to understand the mechanisms by which BPV-1 induces sarcoids, we used gene expression profiling as a screening tool to identify candidate genes implicated in disease pathogenesis. Gene expression profiles of equine fibroblasts transformed by BPV-1 experimentally or from explanted tumors were compared with those of control equine fibroblasts to identify genes associated with expression of BPV-1. Analysis of the microarray data identified 81 probe sets that were significantly (P < 0.01) differentially expressed between the BPV-1-transformed and control cell lines. Expression of several deregulated genes, including MMP-1, CXCL5, FRA-1, NKG7, TLR4, and the gene encoding the major histocompatibility complex class I (MHC-I) protein, was confirmed using other BPV-1-transformed cell lines. Furthermore, expression of these genes was examined using a panel of 10 sarcoids. Increased expression of MMP-1, CXCL5, FRA-1, and NKG7 was detected in a subset of tumors, and TLR4 and MHC I showed robust down-regulation in all tumors. Deregulated expression was confirmed at the protein level for MMP-1 and MHC-1. The present report identifies genes modulated by BPV-1 transformation and will help identify the molecular mechanisms involved in disease pathogenesis.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Gault, Mrs Elizabeth and Campo, Professor Saveria and Nasir, Professor Lubna and Marchetti, Dr Barbara and Yuan, Dr Zhengqiang and Nicolson, Dr Lesley |
| Authors: | Yuan, Z.Q., Nicolson, L., Marchetti, B., Gault, E.A., Campo, M.S., and Nasir, L. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
| Journal Name: | Journal of Virology |
| Journal Abbr.: | J. virol. |
| ISSN: | 0022-538X |
| ISSN (Online): | 1098-5514 |
| Published Online: | 23 April 2008 |
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