Lab-on-a-chip technologies for proteomic analysis from isolated cells

Sedgwick, H., Caron, F., Monaghan, P. B., Kolch, W. and Cooper, J. M. (2008) Lab-on-a-chip technologies for proteomic analysis from isolated cells. Journal of the Royal Society: Interface, 5, S123-S130. (doi:10.1098/rsif.2008.0169.focus)

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Publisher's URL: http://dx.doi.org/10.1098/rsif.2008.0169.focus

Abstract

Lab-on-a-chip systems offer a versatile environment in which low numbers of cells and molecules can be manipulated, captured, detected and analysed. We describe here a micro-fluidic device that allows the isolation, electroporation and lysis of single cells. A431 human epithelial carcinoma cells, expressing a green fluorescent protein-labelled actin, were trapped by dielectrophoresis within an integrated lab-on-a-chip device containing saw-tooth micro-electrodes. Using these same trapping electrodes, on-chip electroporation was performed, resulting in cell lysis. Protein release was monitored by confocal fluorescence microscopy

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Caron, Dr Francois and Kolch, Prof Walter and Monaghan, Dr Paul and Cooper, Professor Jonathan
Authors: Sedgwick, H., Caron, F., Monaghan, P. B., Kolch, W., and Cooper, J. M.
College/School:College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Journal of the Royal Society: Interface
ISSN:1742-5689

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