Quantitative comparison between microfluidic and microtiter plate formats for cell-based assays

Yin, H. , Pattrick, N., Zhang, X.L., Klauke, N., Cordingley, H.C., Haswell, S.J. and Cooper, J.M. (2008) Quantitative comparison between microfluidic and microtiter plate formats for cell-based assays. Analytical Chemistry, 80(1), pp. 179-185. (doi:10.1021/ac701958z)

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Publisher's URL: http://dx.doi.org/10.1021/ac701958z

Abstract

In this paper, we compare a quantitative cell-based assay measuring the intracellular Ca2+ response to the agonist uridine 5'-triphosphate in Chinese hamster ovary cells, in both microfluidic and microtiter formats. The study demonstrates that, under appropriate hydrodynamic conditions, there is an excellent agreement between traditional well-plate assays and those obtained on-chip for both suspended immobilized cells and cultured adherent cells. We also demonstrate that the on-chip assay, using adherent cells, provides the possibility of faster screening protocols with the potential for resolving subcelluar information about local Ca2+ flux

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Klauke, Dr Norbert and Yin, Professor Huabing and Cooper, Professor Jonathan
Authors: Yin, H., Pattrick, N., Zhang, X.L., Klauke, N., Cordingley, H.C., Haswell, S.J., and Cooper, J.M.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Analytical Chemistry
Journal Abbr.:Anal. chem.
ISSN:0003-2700
ISSN (Online):1520-6882
Published Online:06 December 2007

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