Characterization of cellular chemical dynamics using combined microfluidic and Raman techniques

Zhang, X.L., Yin, H.B. , Cooper, J.M. and Haswell, S.J. (2008) Characterization of cellular chemical dynamics using combined microfluidic and Raman techniques. Analytical and Bioanalytical Chemistry, 390(3), pp. 833-840. (doi:10.1007/s00216-007-1564-9)

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Publisher's URL: http://dx.doi.org/10.1007/s00216-007-1564-9

Abstract

The integration of a range of technologies including microfluidics, surface-enhanced Raman scattering and confocal microspectroscopy has been successfully used to characterize in situ single living CHO (Chinese hamster ovary) cells with a high degree of spatial (in three dimensions) and temporal (1 s per spectrum) resolution. Following the introduction of a continuous flow of ionomycin, the real time spectral response from the cell was monitored during the agonist-evoked Ca2+ flux process. The methodology described has the potential to be used for the study of the cellular dynamics of a range of signalling processes

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Yin, Professor Huabing and Cooper, Professor Jonathan
Authors: Zhang, X.L., Yin, H.B., Cooper, J.M., and Haswell, S.J.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Analytical and Bioanalytical Chemistry
Journal Abbr.:Anal. bioanal. chem.
ISSN:1618-2642
ISSN (Online):1618-2650
Published Online:12 September 2007

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