A hierarchical response of cells to perpendicular micro- and nanometric textural cues

Seunarine, K., Curtis, A.S.G., Meredith, D.O., Wilkinson, C.D.W., Riehle, M.O. and Gadegaard, N. (2009) A hierarchical response of cells to perpendicular micro- and nanometric textural cues. IEEE Transactions on NanoBioscience, 8(3), pp. 219-225. (doi:10.1109/TNB.2009.2016477)

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Publisher's URL: http://dx.doi.org/10.1109/TNB.2009.2016477

Abstract

In this paper, we report on the influence of shallow micro-and nanopatterned substrata on the attachment and behavior of a human fibroblast [human telomerase transfected immortalized (hTERT)] cells. We identify a hierarchy of textural guidance cues with respect to cell alignment on these substrates. Cells were seeded and cultured for 48 h on silicon substrates patterned with two linear textures overlaid at 90 degrees, both with 24 mu m pitch: a micrograting and a nanopattern of rows of 140-nm-diameter pits arranged in a rectangular array with 300 nm centre-to-centre spacing. The cell response to these textures was shown to be highly dependent on textural feature dimensions. We show that cells align to the stripes of nanopits. Stripes of 30-nm deep nanopits were also shown to elicit a stronger response from cells than 160-nm deep nanopits.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Curtis, Professor Adam and Seunarine, Dr Krishna and Riehle, Dr Mathis and Wilkinson, Professor Christopher and Gadegaard, Professor Nikolaj
Authors: Seunarine, K., Curtis, A.S.G., Meredith, D.O., Wilkinson, C.D.W., Riehle, M.O., and Gadegaard, N.
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering > School of Engineering
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:IEEE Transactions on NanoBioscience
ISSN:1536-1241
ISSN (Online):1558-2639
Published Online:10 March 2009

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