Biodegradable microgrooved polymeric surfaces obtained by photolithography for skeletal muscle cell orientation and myotube development

Altomare, L., Gadegaard, N. , Visai, L., Tanzi, M.C. and Fare, S. (2010) Biodegradable microgrooved polymeric surfaces obtained by photolithography for skeletal muscle cell orientation and myotube development. Acta Biomaterialia, 6(6), pp. 1948-1957. (doi:10.1016/j.actbio.2009.12.040)

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During tissue formation, skeletal muscle precursor cells fuse together to form multinucleated myotubes. To understand this mechanism, in vitro systems promoting cell alignment need to be developed; for this purpose, micrometer-scale features obtained on substrate surfaces by photolithography can be used to control and affect cell behaviour. This work was aimed at investigating how differently microgrooved polymeric surfaces can affect myoblast alignment, fusion and myotube formation in vitro. Microgrooved polymeric films were obtained by solvent casting of a biodegradable poly-L-lactide/trimethylene carbonate copolymer (PLLA-TMC) onto microgrooved silicon wafers with different groove widths (5, 10, 25, 50, 100 mu m) and depths (0.5, 1, 2.5, 5 mu m), obtained by a standard photolithographic technique. The surface topography of wafers and films was evaluated by scanning electron microscopy. Cell assays were performed using C2C12 cells and myotube formation was analysed by immunofluorescence assays. Cell alignment and circularity were also evaluated using ImageJ software. The obtained results confirm the ability of microgrooved surfaces to influence myotube formation and alignment; in addition, they represent a novel further improvement to the comprehension of best features to be used. The most encouraging results were observed in the case of microstructured PLLA-TMC films with grooves of 2.5 and 1 mu m depth, presenting, in particular, a groove width of 50 and 25 mu m.

Item Type:Articles
Keywords:Adhesion, alignment, contact guidance, contractile, differentiation, expression, in-vitro, microfabrication, micropatterns, myoblast alignment, myotube formation, PLLA-TMC, scaffolds, topography
Glasgow Author(s) Enlighten ID:Gadegaard, Professor Nikolaj
Authors: Altomare, L., Gadegaard, N., Visai, L., Tanzi, M.C., and Fare, S.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Acta Biomaterialia
ISSN (Online):1878-7568
Published Online:28 December 2009

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