Dynamic changes in lung microRNA profiles during the development of pulmonary hypertension due to chronic hypoxia and monocrotaline

Caruso, P. et al. (2010) Dynamic changes in lung microRNA profiles during the development of pulmonary hypertension due to chronic hypoxia and monocrotaline. Arteriosclerosis, Thrombosis, and Vascular Biology, 30(4), pp. 716-723. (doi: 10.1161/ATVBAHA.109.202028)

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Abstract

<b>Objective</b>: MicroRNAs (miRNAs) are small noncoding RNAs that have the capacity to control protein production through binding "seed" sequences within a target mRNA. Each miRNA is capable of potentially controlling hundreds of genes. The regulation of miRNAs in the lung during the development of pulmonary arterial hypertension (PAH) is unknown.<p></p> <b>Methods and Results</b>: We screened lung miRNA profiles in a longitudinal and crossover design during the development of PAH caused by chronic hypoxia or monocrotaline in rats. We identified reduced expression of Dicer, involved in miRNA processing, during the onset of PAH after hypoxia. MiR-22, miR-30, and let-7f were downregulated, whereas miR-322 and miR-451 were upregulated significantly during the development of PAH in both models. Differences were observed between monocrotaline and chronic hypoxia. For example, miR-21 and let-7a were significantly reduced only in monocrotaline-treated rats. MiRNAs that were significantly regulated were validated by quantitative polymerase chain reaction. By using in vitro studies, we demonstrated that hypoxia and growth factors implicated in PAH induced similar changes in miRNA expression. Furthermore, we confirmed miR-21 downregulation in human lung tissue and serum from patients with idiopathic PAH.<p></p> <b>Conclusion</b>: Defined miRNAs are regulated during the development of PAH in rats. Therefore, miRNAs may contribute to the pathogenesis of PAH and represent a novel opportunity for therapeutic intervention.<p></p>

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Robertson, Dr Keith and MacLean, Professor Margaret and Baker, Professor Andrew and Greig, Ms Jenny and Khanin, Dr Raya and Dempsie, Dr Yvonne and Shirley, Dr Rachel and Denby, Dr Laura and Caruso, Ms Paola and McClure, Dr John
Authors: Caruso, P., MacLean, M. R., Khanin, R., McClure, J., Soon, E., Southgate, M., MacDonald, R. A., Greig, J. A., Robertson, K. E., Masson, R., Denby, L., Dempsie, Y., Long, L., Morrell, N. W., and Baker, A. H.
Subjects:R Medicine > RC Internal medicine
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
College of Medical Veterinary and Life Sciences > Institute of Health and Wellbeing > Robertson Centre
Journal Name:Arteriosclerosis, Thrombosis, and Vascular Biology
Publisher:Lippincott Williams & Wilkins
ISSN:1079-5642
ISSN (Online):1524-4636
Published Online:28 January 2010
Copyright Holders:Copyright © 2010 American Heart Association
First Published:First published in Arteriosclerosis, Thrombosis, and Vascular Biology 30(4):716-723
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher
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