Alvarez-Curto, E., Ward, R. J., Pediani, J. D. and Milligan, G. (2010) Ligand regulation of the quaternary organization of cell surface M3 muscarinic acetylcholine receptors analyzed by fluorescence resonance energy transfer (FRET) imaging and homogenous time-resolved FRET. Journal of Biological Chemistry, 285(30), pp. 23318-23330. (doi: 10.1074/jbc.M110.122184) (PMID:20489201) (PMCID:PMC2906324)
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Publisher's URL: http://dx.doi.org/10.1074/jbc.M110.122184
Abstract
Flp-In T-REx 293 cells expressing a wild type human M muscarinic acetylcholine receptor construct constitutively and able to express a Receptor Activated Solely by Synthetic Ligand (RASSL) form of this receptor on demand maintained response to the muscarinic agonist carbachol but developed response to clozapine-N-oxide only upon induction of the RASSL. The two constructs co-localized at the plasma membrane and generated strong ratiometric fluorescence resonance energy transfer (FRET) signals consistent with direct physical interactions. Increasing levels of induction of the FRET-donor RASSL did not alter wild type receptor FRET-acceptor levels substantially. However, ratiometric FRET was modulated in a bell-shaped fashion with maximal levels of the donor resulting in decreased FRET. Carbachol, but not the antagonist atropine, significantly reduced the FRET signal. Cell surface homogenous time-resolved FRET, based on SNAP-tag technology and employing wild type and RASSL forms of the human M receptor expressed stably in Flp-In TREx 293 cells, also identified cell surface dimeric/oligomeric complexes. Now, however, signals were enhanced by appropriate selective agonists. At the wild type receptor large increases in FRET signal to carbachol and acetylcholine were concentration-dependent with EC values consistent with the relative affinities of the two ligands. These studies confirm the capacity of the human M muscarinic acetylcholine receptor to exist as dimeric/oligomeric complexes at the surface of cells and demonstrate that the organization of such complexes can be modified by ligand binding. However, conclusions as to the effect of ligands on such complexes may depend on the approach used.
| Item Type: | Articles |
|---|---|
| Additional Information: | This research was originally published in Journal of Biological Chemistry. Elisa Alvarez-Curto, Richard J. Ward, John D. Pediani and Graeme Milligan. Ligand regulation of the quaternary organization of cell surface M3 muscarinic acetylcholine receptors analyzed by fluorescence resonance energy transfer (FRET) imaging and homogenous time-resolved FRET. Journal of Biological Chemistry. 2010. 285:23318-23330. © The American Society for Biochemistry and Molecular Biology. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Pediani, Dr John and Ward, Dr Richard and Alvarez-Curto, Dr Elisa and Milligan, Professor Graeme |
| Authors: | Alvarez-Curto, E., Ward, R. J., Pediani, J. D., and Milligan, G. |
| Subjects: | Q Science > QH Natural history > QH345 Biochemistry |
| College/School: | College of Medical Veterinary and Life Sciences > School of Psychology & Neuroscience |
| Journal Name: | Journal of Biological Chemistry |
| Journal Abbr.: | J Biol Chem. |
| Publisher: | American Society for Biochemistry and Molecular Biology, Inc. |
| ISSN: | 0021-9258 |
| ISSN (Online): | 1083-351X |
| Published Online: | 19 May 2010 |
| Copyright Holders: | Copyright © 2010 American Society for Biochemistry and Molecular Biology |
| First Published: | First published in Journal of Biological Chemistry 285(30):23318-23330 |
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