Modulation of NF-kappaB activation in Theileria annulata-infected cloned cell lines is associated with detection of parasite-dependent IKK signalosomes and disruption of the actin cytoskeleton

Schmuckli-Maurer, J., Kinnaird, J., Pillai, S., Hermann, P., McKellar, S., Weir, W. , Dobbelaere, D. and Shiels, B. (2010) Modulation of NF-kappaB activation in Theileria annulata-infected cloned cell lines is associated with detection of parasite-dependent IKK signalosomes and disruption of the actin cytoskeleton. Cellular Microbiology, 12(2), pp. 158-173. (doi:10.1111/j.1462-5822.2009.01386.x) (PMID:19804486)

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Abstract

Apicomplexan parasites within the genus <i>Theileria</i> have the ability to induce continuous proliferation and prevent apoptosis of the infected bovine leukocyte. Protection against apoptosis involves constitutive activation of the bovine transcription factor NF-kappaB in a parasite-dependent manner. Activation of NF-kappaB is thought to involve recruitment of IKK signalosomes at the surface of the macroschizont stage of the parasite, and it has been postulated that additional host proteins with adaptor or scaffolding function may be involved in signalosome formation. In this study two clonal cell lines were identified that show marked differences in the level of activated NF-kappaB. Further characterization of these lines demonstrated that elevated levels of activated NF-kappaB correlated with increased resistance to cell death and detection of parasite-associated IKK signalosomes, supporting results of our previous studies. Evidence was also provided for the existence of host- and parasite-dependent NF-kappaB activation pathways that are influenced by the architecture of the actin cytoskeleton. Despite this influence, it appears that the primary event required for formation of the parasite-dependent IKK signalosome is likely to be an interaction between a signalosome component and a parasite-encoded surface ligand.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Weir, Dr William and Shiels, Professor Brian and Kinnaird, Dr Jane
Authors: Schmuckli-Maurer, J., Kinnaird, J., Pillai, S., Hermann, P., McKellar, S., Weir, W., Dobbelaere, D., and Shiels, B.
Subjects:Q Science > QR Microbiology
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Cellular Microbiology
Publisher:Wiley-Blackwell
ISSN:1462-5814
ISSN (Online):1462-5822
Published Online:05 October 2009
Copyright Holders:Copyright © 2010 Wiley-Blackwell
First Published:First published in Cellular Microbiology 12(2):158-173
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
392992An integrated approach for the development of sustainable methods to control tropical theileriosisAndrew TaitWellcome Trust (WELLCOTR)075820/A/04/ZSCHOOL OF VETERINARY MEDICINE