Manipulation of live mouse embryonic stem cells using holographic optical tweezers

Leach, J., Howard, D., Roberts, S., Gibson, G., Gothard, D., Cooper, J. , Shakesheff, K., Padgett, M. and Buttery, L. (2009) Manipulation of live mouse embryonic stem cells using holographic optical tweezers. Journal of Modern Optics, 56(4), pp. 448-452. (doi:10.1080/09500340802488565)

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Abstract

We report the ability to move and arrange patterns of live embryonic stem cells using holographic optical tweezers. Single cell suspensions of mouse embryonic stem cells were manipulated with holographic optical tweezers into a variety of patterns including lines, curves and circles. Individual cells were also lifted out of the sample plane highlighting the potential for 3D positional control. Trypan blue dye exclusion and Live/Dead™ staining (CMFDA-1, EthHD-1) showed that the cells were still viable after manipulation with the optical tweezers. The ability to move individual stem cells into specific, pre-defined patterns provides a method to study how arrangement and associated small-scale interactions occur between neighbouring cells.

Item Type:Articles
Keywords:4-cell stage, cell, cells, England, in-vitro differentiation, lines, manipulation, optical tweezers, optics, particles, spatial light modulators, stem cells, stem-cells, traps, tweezers
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Gibson, Dr Graham and Padgett, Professor Miles and Leach, Mr Jonathan and Cooper, Professor Jonathan
Authors: Leach, J., Howard, D., Roberts, S., Gibson, G., Gothard, D., Cooper, J., Shakesheff, K., Padgett, M., and Buttery, L.
College/School:College of Science and Engineering > School of Physics and Astronomy
College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Journal of Modern Optics
Journal Abbr.:J. mod. opt.
ISSN:0950-0340
ISSN (Online):1362-3044

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