Manipulation of live mouse embryonic stem cells using holographic optical tweezers

Leach, J., Howard, D., Roberts, S., Gibson, G. , Gothard, D., Cooper, J. , Shakesheff, K., Padgett, M. and Buttery, L. (2009) Manipulation of live mouse embryonic stem cells using holographic optical tweezers. Journal of Modern Optics, 56(4), pp. 448-452. (doi: 10.1080/09500340802488565)

Abstract

We report the ability to move and arrange patterns of live embryonic stem cells using holographic optical tweezers. Single cell suspensions of mouse embryonic stem cells were manipulated with holographic optical tweezers into a variety of patterns including lines, curves and circles. Individual cells were also lifted out of the sample plane highlighting the potential for 3D positional control. Trypan blue dye exclusion and Live/Dead™ staining (CMFDA-1, EthHD-1) showed that the cells were still viable after manipulation with the optical tweezers. The ability to move individual stem cells into specific, pre-defined patterns provides a method to study how arrangement and associated small-scale interactions occur between neighbouring cells.

• Item Type: Articles
• Keywords: 4-cell stage, cell, cells, England, in-vitro differentiation, lines, manipulation, optical tweezers, optics, particles, spatial light modulators, stem cells, stem-cells, traps, tweezers
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Gibson, Dr Graham and Padgett, Professor Miles and Leach, Mr Jonathan and Cooper, Professor Jonathan
• Authors: Leach, J., Howard, D., Roberts, S., Gibson, G., Gothard, D., Cooper, J., Shakesheff, K., Padgett, M., and Buttery, L.
• College/School: College of Science and Engineering > School of Physics and Astronomy; College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering; College of Science and Engineering > School of Engineering > Biomedical Engineering
• Journal Name: Journal of Modern Optics
• Journal Abbr.: J. mod. opt.
• ISSN: 0950-0340
• ISSN (Online): 1362-3044