Nanometric surface patterns for tissue engineering: fabrication and biocompatibility in vitro

Riehle, M., Dalby, M., Johnstone, H., Gallagher, J., Wood, M.A., Casey, B., Mcghee, K., Affrossman, S., Wilkinson, C.D.W. and Curtis, A.S.G. (2002) Nanometric surface patterns for tissue engineering: fabrication and biocompatibility in vitro. In: Nanopatterning From Ultralarge Scale.Integration to Biotechnology Symposium Materials Research Society, Warrendale, USA, pp. 237-246. ISBN 1558996419 (doi: 10.1557/PROC-705-Y5.1)

Abstract

Three fundamentally different methods were used to fabricate nanometric surface features on polymers or fused silica. Phase separation of binary polymer mixes resulted in randomly distributed features whose depth and shape could be tightly controlled over large areas. Colloidal resist patterned large areas randomly and uniformly with very fine spikes. In contrast e-beam and reactive ion etching were used to create a set of regular spaced pillars on an orthogonal pattern. Some of the surfaces were replicated by in situ polymerization, solvent casting, embossing or melt molding onto polystyrene (PS) or ε–poly caprolactone (ε–PCL). Nanometric features down to 60nm were imprinted onto the polymers with high fidelity. Cells were seeded onto the nanometric surfaces and adhesion, morphology and cytoskeleton investigated. Cells respond to regular features of 170/80nm (width/depth) with reduced adhesion and changes in overall morphology and cytoskeleton. Small nanofeatures (13nm, 35nm depth) made by phase separation on the other hand increased adhesion and promoted cytoskeletal differentiation. The responses of the cells are indicative that nanometric surface features are useful modifications on scaffolds for tissue engineering or on medical implants.

• Item Type: Conference Proceedings
• Keywords: Biocompatibility, Engineering, Fabrication, In-Vitro, Nanopatterning.From.Ultralarge.Scale.Integration.To.Biotechnology, Surface, Tissue, Tissue Engineering
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Wilkinson, Professor Christopher
• Authors: Riehle, M., Dalby, M., Johnstone, H., Gallagher, J., Wood, M.A., Casey, B., Mcghee, K., Affrossman, S., Wilkinson, C.D.W., and Curtis, A.S.G.
• College/School: College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering
• Publisher: Material research society
• ISBN: 1558996419