Similarities and differences between the E5 oncoproteins of bovine papillomaviruses type 1 and type 4: Cytoskeleton, motility and invasiveness in E5-transformed bovine and mouse cells

Tsirimonaki, E., Ullah, R., Marchetti, B., Ashrafi, G.H., McGarry, L., Ozanne, B.W. and Campo, M.S. (2006) Similarities and differences between the E5 oncoproteins of bovine papillomaviruses type 1 and type 4: Cytoskeleton, motility and invasiveness in E5-transformed bovine and mouse cells. Virus Research, 115(2), pp. 158-168. (doi: 10.1016/j.virusres.2005.08.003)

Abstract

Bovine papillomaviruses (BPVs) are oncogenic viruses. In cattle, BPV-1/2 is associated with urinary bladder cancer and BPV-4 with upper GI tract cancer. BPV E5 is a small hydrophobic protein localised in the endoplasmic reticulum (ER) and Golgi apparatus (GA). E5 is the major transforming protein of BPVs, capable of inducing cell transformation in cultured mouse fibroblasts and, in cooperation with E7, in primary bovine cells. E5-induced cell transformation is accompanied by activation of several cellular protein kinases, including growth factor receptors, and alkalinisation of endosomes and GA. We have reported that BPV E5 causes swelling and fragmentation of the GA and extensive vacuolisation of the cytoplasm. We now show that E5 from both BPV-1 and BPV-4 disturbs the actin cytoskeleton and focal adhesions in transformed bovine cells, where these morphological and behavioural characteristics are accompanied by hyperphosphorylation of the cellular phosphotyrosine kinase c-src. Both BPV-1 and BPV-4 E5 increase the motility of transformed mouse cells, but only BPV-1 E5 causes transformed mouse cells to penetrate a matrigel matrix. BPV-1 transformed mouse cells, but not BPV-4 transformed mouse cells, have hyperhpsphorylated c-src.

• Item Type: Articles
• Keywords: BPV, E5, Cell transformation, Cytoskeleton, Focal adhesions, Motility, Invasion, c-src.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Ashrafi, Dr Gholam Hossein and Campo, Professor Saveria and Marchetti, Dr Barbara and McGarry, Ms Lynn and Ozanne, Professor Bradford
• Authors: Tsirimonaki, E., Ullah, R., Marchetti, B., Ashrafi, G.H., McGarry, L., Ozanne, B.W., and Campo, M.S.
• Subjects: S Agriculture > SF Animal culture > SF600 Veterinary Medicine; R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer); Q Science > QR Microbiology > QR355 Virology
• College/School: College of Medical Veterinary and Life Sciences
• Journal Name: Virus Research
• Publisher: Elsevier Science
• ISSN: 0168-1702
• Copyright Holders: Copyright © 2006 Elsevier Science
• First Published: First published in Virus Research 115(2):158-168
• Publisher Policy: Reproduced in accordance with the copyright policy of the publisher