The E5 protein of BPV-4 interacts with the heavy chain of MHC class I and irreversibly retains the MHC complex in the Golgi apparatus

Marchetti, B., Ashrafi, G.H., Dornan, E.S., Araibi, E.H., Ellis, S.A. and Campo, M.S. (2006) The E5 protein of BPV-4 interacts with the heavy chain of MHC class I and irreversibly retains the MHC complex in the Golgi apparatus. Oncogene, 25(15), pp. 2254-2263. (doi: 10.1038/sj.onc.1209245)

Abstract

BPV-4 E5 inhibits transcription of the bovine MHC class I heavy chain (HC) gene, increases degradation of HC and downregulates surface expression of MHC class I by retaining the complex in the Golgi apparatus (GA). Here we report that transcription inhibition can be alleviated by interferon treatment and the degradation of HC can be reversed by treatment with inhibitors of proteasomes and lysosomes. However, the inhibition of transport of MHC class I to the cell surface is irreversible. We show that E5 is capable of physically interacting with HC. Together with the inhibition of the vacuolar ATPase (due to the interaction between E5 and 16k subunit c), the interaction between E5 and HC is likely to be responsible for retention of MHC class I in the GA. C-terminus deletion mutants of E5 are incapable of either downregulating surface MHC class I or interacting with HC, establishing that the C-terminus domain of E5 is important in the inhibition of MHC class I.

• Item Type: Articles
• Keywords: BPV-4, E5, MHC class I, heavy chain, interaction, immunoevasion.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Ashrafi, Dr Gholam Hossein and Campo, Professor Saveria and Marchetti, Dr Barbara and Dornan, Mr Edward
• Authors: Marchetti, B., Ashrafi, G.H., Dornan, E.S., Araibi, E.H., Ellis, S.A., and Campo, M.S.
• Subjects: Q Science > QR Microbiology > QR180 Immunology; S Agriculture > SF Animal culture > SF600 Veterinary Medicine; R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer); Q Science > QH Natural history
• College/School: College of Medical Veterinary and Life Sciences; College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
• Journal Name: Oncogene
• Publisher: Nature Publishing Group
• ISSN: 0950-9232
• Copyright Holders: Copyright © 2006 Nature Publishing Group
• First Published: First published in Oncogene 25(15):2254-2263
• Publisher Policy: Reproduced in accordance with the copyright policy of the publisher