The E5 protein of BPV-4 interacts with the heavy chain of MHC class I and irreversibly retains the MHC complex in the Golgi apparatus

Marchetti, B., Ashrafi, G.H., Dornan, E.S., Araibi, E.H., Ellis, S.A. and Campo, M.S. (2006) The E5 protein of BPV-4 interacts with the heavy chain of MHC class I and irreversibly retains the MHC complex in the Golgi apparatus. Oncogene, 25(15), pp. 2254-2263. (doi:10.1038/sj.onc.1209245)

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Publisher's URL: http://dx.doi.org/10.1038/sj.onc.1209245

Abstract

BPV-4 E5 inhibits transcription of the bovine MHC class I heavy chain (HC) gene, increases degradation of HC and downregulates surface expression of MHC class I by retaining the complex in the Golgi apparatus (GA). Here we report that transcription inhibition can be alleviated by interferon treatment and the degradation of HC can be reversed by treatment with inhibitors of proteasomes and lysosomes. However, the inhibition of transport of MHC class I to the cell surface is irreversible. We show that E5 is capable of physically interacting with HC. Together with the inhibition of the vacuolar ATPase (due to the interaction between E5 and 16k subunit c), the interaction between E5 and HC is likely to be responsible for retention of MHC class I in the GA. C-terminus deletion mutants of E5 are incapable of either downregulating surface MHC class I or interacting with HC, establishing that the C-terminus domain of E5 is important in the inhibition of MHC class I.

Item Type:Articles
Keywords:BPV-4, E5, MHC class I, heavy chain, interaction, immunoevasion.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Ashrafi, Dr Gholam Hossein and Campo, Professor Saveria and Marchetti, Dr Barbara and Dornan, Mr Edward
Authors: Marchetti, B., Ashrafi, G.H., Dornan, E.S., Araibi, E.H., Ellis, S.A., and Campo, M.S.
Subjects:Q Science > QR Microbiology > QR180 Immunology
S Agriculture > SF Animal culture > SF600 Veterinary Medicine
R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Q Science > QH Natural history
College/School:College of Medical Veterinary and Life Sciences
Journal Name:Oncogene
Publisher:Nature Publishing Group
ISSN:0950-9232
Copyright Holders:Copyright © 2006 Nature Publishing Group
First Published:First published in Oncogene 25(15):2254-2263
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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