Abundant variation in microsatellites of the parasitic nematode Trichostrongylus tenuis and linkage to a tandem repeat

Johnson, P.C.D. , Webster, L.M.I., Adam, A., Buckland, R., Dawson, D.A. and Keller, L.F. (2006) Abundant variation in microsatellites of the parasitic nematode Trichostrongylus tenuis and linkage to a tandem repeat. Molecular and Biochemical Parasitology, 148(2), pp. 210-218. (doi:10.1016/j.molbiopara.2006.04.011)

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Abstract

An understanding of how genes move between and within populations of parasitic nematodes is important in combating the evolution and spread of anthelmintic resistance. Much has been learned by studying mitochondrial DNA markers, but autosomal markers such as microsatellites have been applied to only a few nematode species, despite their many advantages for studying gene flow in eukaryotes. Here, we describe the isolation of 307 microsatellites from Trichostrongylus tenuis, an intestinal nematode of red grouse. High levels of variation were revealed at sixteen microsatellite loci (including three sex-lined loci) in 111 male T. tenuis nematodes collected from four hosts at a single grouse estate in Scotland (average He = 0.708; mean number of alleles = 12.2). A population genetic analysis detected no deviation from panmixia either between (F(ST) = 0.00) or within hosts (F(IS) = 0.015). We discuss the feasibility of developing microsatellites in parasitic nematodes and the problem of null alleles. We also describe a novel 146-bp repeat element, TteREP1, which is linked to two-thirds of the microsatellites sequenced and is associated with marker development failure. The sequence of TteREP1 is related to the TcREP-class of repeats found in several other trichostrongyloid species including Trichostrongylus colubriformis and Haemonchus contortus.

Item Type:Articles
Keywords:Microsatellite; Parasitic nematode; Population genetics; Repetitive elements; Sex-linkage; Trichostronglylus tenuis
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Johnson, Dr Paul and Keller, Prof Lukas
Authors: Johnson, P.C.D., Webster, L.M.I., Adam, A., Buckland, R., Dawson, D.A., and Keller, L.F.
Subjects:Q Science > QL Zoology
Q Science > QH Natural history > QH426 Genetics
College/School:College of Medical Veterinary and Life Sciences > Institute of Health and Wellbeing > Robertson Centre
Journal Name:Molecular and Biochemical Parasitology
Publisher:Elsevier BV
ISSN:0166-6851
ISSN (Online):1872-9428
Published Online:16 May 2006
Copyright Holders:Copyright © 2006 Elsevier Science
First Published:First published in Molecular and Biochemical Parasitology 148(2):210-218
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
301131Evolutionary Consequences of Population Genetic Structure in a Parasitic NematodeLukas KellerNatural Environment Research Council (NERC)NER/M/S/2001/00SCHOOL OF LIFE SCIENCES