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Suppression of virus burden by immunization with feline immunodeficiency virus Env protein

Hosie, M.J., Dunsford, T.H., de Ronde, A., Willett, B.J., Cannon, C.A., Neil, J.C., and Jarrett, O. (1996) Suppression of virus burden by immunization with feline immunodeficiency virus Env protein. Vaccine, 14 (5). pp. 405-411. ISSN 0264-410X (doi:10.1016/0264-410X(95)00193-5)

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Abstract

Whole inactivated virus (WIV) vaccines derived from the FLA cell line protect cats against challenge with feline immunodeficiency virus (FIV). To discover whether the protective effects of WIV could be reproduced by the isolated Env component, either WIV or immunoaffinity-purified FIV gp120 from the FL4 cell line was administered to cats. Although both vaccines induced high levels of virus neutralizing antibodies, purified Env was much less effective than WIV in protecting cats against viraemia. However, reduced virus load in PBMCs was evident in all Env-immunized cats compared to controls. Analyses of antibody responses to bacterial expression products of FIV Env, which were high in Env-immunized cats but low in animals receiving the WIV vaccine, suggested that the partially denatured, monomeric Env induces a less effective antiviral immune response than WIV. Hence, the superior immunogenicity of WIV may be due to the presentation of the native oligomeric structure of Env on virions.

Item Type:Article
Status:Published
Refereed:Yes
Glasgow Author(s):Hosie, Prof Margaret and Willett, Prof Brian and Cannon, Mrs Cecilia and Neil, Prof James
Authors: Hosie, M.J., Dunsford, T.H., de Ronde, A., Willett, B.J., Cannon, C.A., Neil, J.C., and Jarrett, O.
Subjects:Q Science > QR Microbiology > QR355 Virology
S Agriculture > SF Animal culture > SF600 Veterinary Medicine
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation > Centre for Virus Research
Journal Name:Vaccine
ISSN:0264-410X
ISSN (Online):1873-2518
Published Online:8 December 1999

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