Effects of FSH on testicular mRNA transcript levels in the hypogonadal mouse

Abel, M.H., Baban, D., Lee, S., Charlton, H.M. and O'Shaughnessy, P.J. (2009) Effects of FSH on testicular mRNA transcript levels in the hypogonadal mouse. Journal of Molecular Endocrinology, 42(4), pp. 291-303. (doi:10.1677/JME-08-0107)

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Abstract

Follicle stimulating hormone (FSH) acts through the Sertoli cell to ensure normal testicular development and function. To identify transcriptional mechanisms through which FSH acts in the testis we have treated gonadotrophin-deficient hypogonadal (hpg) mice with recombinant FSH and measured changes in testicular transcript levels using microarrays and real-time PCR 12, 24 and 72h after the start of treatment. Approximately 400 transcripts were significantly altered at each time point by FSH treatment. At 12h there was a clear increase in the levels of a number of transcripts known to be expressed in the Sertoli cells (eg Fabp5, Lgals1, Tesc, Scara5, Aqp5). Additionally, level of Leydig cell transcipts were also markedly increased (eg Ren1, Cyp17a1, Akr1b7, Star, Nr4a1). This was associated with a small but significant rise in testosterone at 24 and 72h. At 24h, androgen-dependent Sertoli cell transcripts were upregulated (eg Rhox5, Drd4, Spinlw1, Tubb3 and Tsx) and this trend continued up to 72h. Surprisingly, only 4 germ cell trancripts (Dkkl1, Hdc, Oct4 and 1700021K02Rik) were altered by FSH within the time-course of the experiment. Pathway analysis showed changes in cell cycle and cell proliferation pathways at all times and a general decline in transcripts related to formation and regulation of tight junctions. Results show FSH acts directly and indirectly to induce rapid changes in Sertoli cell and Leydig cell transcript levels in the hpg mouse but that effects on germ cell development must occur over a longer time span.

Item Type:Articles (Other)
Additional Information:"Disclaimer. This is not the definitive version of record of this article. This manuscript has been accepted for publication in Journal of Molecular Endocrinology, but the version presented here has not yet been copy edited, formatted or proofed. Consequently, the Society for Endocrinology accepts no responsibility for any errors or omissions it may contain. The definitive version is now freely available at http://dx.doi.org/10.1677/JME-08-0107. © 2009 Society for Endocrinology."
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Lee, Ms Sylvia and O'Shaughnessy, Professor Peter
Authors: Abel, M.H., Baban, D., Lee, S., Charlton, H.M., and O'Shaughnessy, P.J.
Subjects:Q Science > QH Natural history > QH345 Biochemistry
Q Science > QP Physiology
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
Journal Name:Journal of Molecular Endocrinology
Journal Abbr.:J. Mol. Endo.
Publisher:Society for Endocrinology
ISSN:0952-5041
ISSN (Online):1479-6813
Published Online:09 January 2009
Copyright Holders:Copyright © 2009 Society for Endocrinology
First Published:First published in Journal of Molecular Endocrinology 42(2):291-303
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
413101Testicular function in mice lacking sertoli cell androgen receptors and FSH receptorsPeter O'ShaughnessyWellcome Trust (WELLCOME)078137/Z/05/ZInstitute of Biodiversity Animal Health and Comparative Medicine