Spermatogenesis and sertoli cell activity in mice lacking Sertoli cell receptors for follicle stimulating hormone and androgen

Abel, M.H., Baker, P.J., Charlton, H.M., Monteiro, A., Verhoeven, G., De Gendt, K., Guillou, F. and O'Shaughnessy, P.J. (2008) Spermatogenesis and sertoli cell activity in mice lacking Sertoli cell receptors for follicle stimulating hormone and androgen. Endocrinology, 149(7), pp. 3279-3285. (doi:10.1210/en.2008-0086)

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Abstract

Spermatogenesis in the adult male depends on the action of FSH and androgen. Ablation of either hormone has deleterious effects on Sertoli cell function and the progression of germ cells through spermatogenesis. In this study we generated mice lacking both FSH receptors (FSHRKO) and androgen receptors on the Sertoli cell (SCARKO) to examine how FSH and androgen combine to regulate Sertoli cell function and spermatogenesis. Sertoli cell number in FSHRKO-SCARKO mice was reduced by about 50% but was not significantly different from FSHRKO mice. In contrast, total germ cell number in FSHRKO-SCARKO mice was reduced to 2% of control mice (and 20% of SCARKO mice) due to a failure to progress beyond early meiosis. Measurement of Sertoli cell-specific transcript levels showed that about a third were independent of hormonal action on the Sertoli cell, whereas others were predominantly androgen dependent or showed redundant control by FSH and androgen. Results show that FSH and androgen act through redundant, additive, and synergistic regulation of spermatogenesis and Sertoli cell activity. In addition, the Sertoli cell retains a significant capacity for activity, which is independent of direct hormonal regulation.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Monteiro, Dr Ana and O'Shaughnessy, Professor Peter and Baker, Dr Paul
Authors: Abel, M.H., Baker, P.J., Charlton, H.M., Monteiro, A., Verhoeven, G., De Gendt, K., Guillou, F., and O'Shaughnessy, P.J.
Subjects:Q Science > QP Physiology
Q Science > QH Natural history > QH301 Biology
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
Journal Name:Endocrinology
Publisher:Endocrine Society
ISSN:0013-7227
ISSN (Online):1945-7170
Published Online:10 April 2008
Copyright Holders:Copyright © 2008 Endocrine Society
First Published:First published in Endocrinology 149(7):3279-3285
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
413101Testicular function in mice lacking sertoli cell androgen receptors and FSH receptorsPeter O'ShaughnessyWellcome Trust (WELLCOME)078137/Z/05/ZInstitute of Biodiversity Animal Health and Comparative Medicine