Genetic diversity of Brazilian isolates of feline immunodeficiency virus

Teixeira, B. M., Logan, N., Cruz, J.C.M., Reis, J.K.P., Brandão, P.E., Richtzenhain, L.J., Hagiwara, M.K., Willett, B.J. and Hosie, M.J. (2010) Genetic diversity of Brazilian isolates of feline immunodeficiency virus. Archives of Virology, 155(3), pp. 379-384. (doi: 10.1007/s00705-009-0587-2)

[img] Text
30383.pdf

97kB

Publisher's URL: http://dx.doi.org/10.1007/s00705-009-0587-2

Abstract

We isolated Feline immunodeficiency virus (FIV) from three adult domestic cats, originating from two open shelters in Brazil. Viruses were isolated from PBMC following co-cultivation with the feline T-lymphoblastoid cell line MYA-1. All amplified env gene products were cloned directly into pGL8MYA. The nucleic acid sequences of seven clones were determined and then compared with those of previously described isolates. The sequences of all of the Brazilian virus clones were distinct and phylogenetic analysis revealed that all belong to subtype B. Three variants isolated from one cat and two variants were isolated from each of the two other cats, indicating that intrahost diversity has the potential to pose problems for the treatment and diagnosis of FIV infection.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Hosie, Professor Margaret and Willett, Professor Brian
Authors: Teixeira, B. M., Logan, N., Cruz, J.C.M., Reis, J.K.P., Brandão, P.E., Richtzenhain, L.J., Hagiwara, M.K., Willett, B.J., and Hosie, M.J.
Subjects:Q Science > QR Microbiology > QR355 Virology
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Archives of Virology
Publisher:Springer Wein
ISSN:0304-8608
ISSN (Online):1432-8798
Published Online:20 January 2010
Related URLs:

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
363381Rational Design of a Lentiviral VaccineMargaret HosieMedical Research Council (MRC)G0300387Centre for Virus Research