Mapping the domains of CD134 as a functional receptor for feline immunodeficiency virus (FIV)

Willett, B.J., McMonagle, E.L., Bonci, F., Pistello, M. and Hosie, M.J. (2006) Mapping the domains of CD134 as a functional receptor for feline immunodeficiency virus (FIV). Journal of Virology, 80(15), pp. 7744-7747. (doi: 10.1128/JVI.00722-06)

Abstract

The feline homologue of CD134 (fCD134) is the primary binding receptor for feline immunodeficiency virus (FIV), targeting the virus preferentially to activated CD4+ helper T cells. However, strains of FIV differ in their utilisation of CD134; the prototypic strain PPR, requires a minimal determinant in CRD1 of fCD134 to confer near optimal receptor function while strains such as GL8 require additional determinants in the CD134 CRD2. We map this determinant to a loop in CRD2 governing the interaction between the receptor and its ligand; substitution of amino acids S78N,S79Y,K80E restored full viral receptor activity to the CDR2 of human CD134 in the context of feline CD134 with tyrosine-79 appearing to be the critical residue for restoration of receptor function.

• Item Type: Articles
• Keywords: AIDS, FIV, HIV receptor
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Hosie, Professor Margaret and Willett, Professor Brian
• Authors: Willett, B.J., McMonagle, E.L., Bonci, F., Pistello, M., and Hosie, M.J.
• Subjects: S Agriculture > SF Animal culture > SF600 Veterinary Medicine
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity; College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
• Research Group: Retrovirus Research Laboratory
• Journal Name: Journal of Virology
• Journal Abbr.: J. Virol.
• Publisher: American Society for Microbiology
• ISSN: 0022-538X
• ISSN (Online): 1098-5514
• Copyright Holders: Copyright © 2006 American Society for Microbiology
• First Published: First published in Journal of Virology 80(15):7744-7747
• Publisher Policy: Reproduced in accordance with the copyright policy of the publisher

Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
36338	1	Rational Design of a Lentiviral Vaccine	Margaret Hosie	Medical Research Council (MRC)	G0300387	Centre for Virus Research