High-speed multi-objective Fourier ptychographic microscopy

Aidukas, T., Konda, P. C. and Harvey, A. R. (2022) High-speed multi-objective Fourier ptychographic microscopy. Optics Express, 30(16), pp. 29189-29205. (doi: 10.1364/OE.466075) (PMID:36299099)

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Abstract

The ability of a microscope to rapidly acquire wide-field, high-resolution images is limited by both the optical performance of the microscope objective and the bandwidth of the detector. The use of multiple detectors can increase electronic-acquisition bandwidth, but the use of multiple parallel objectives is problematic since phase coherence is required across the multiple apertures. We report a new synthetic-aperture microscopy technique based on Fourier ptychography, where both the illumination and image-space numerical apertures are synthesized, using a spherical array of low-power microscope objectives that focus images onto mutually incoherent detectors. Phase coherence across apertures is achieved by capturing diffracted fields during angular illumination and using ptychographic reconstruction to synthesize wide-field, high-resolution, amplitude and phase images. Compared to conventional Fourier ptychography, the use of multiple objectives reduces image acquisition times by increasing the area for sampling the diffracted field. We demonstrate the proposed scaleable architecture with a nine-objective microscope that generates an 89-megapixel, 1.1 µm resolution image nine-times faster than can be achieved with a single-objective Fourier-ptychographic microscope. New calibration procedures and reconstruction algorithms enable the use of low-cost 3D-printed components for longitudinal biological sample imaging. Our technique offers a route to high-speed, gigapixel microscopy, for example, imaging the dynamics of large numbers of cells at scales ranging from sub-micron to centimetre, with an enhanced possibility to capture rare phenomena.

Item Type:Articles
Additional Information:Funding: Engineering and Physical Sciences Research Council (EP/L016753/1).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Konda, Mr Pavan and Aidukas, Dr Tomas and Harvey, Professor Andy
Authors: Aidukas, T., Konda, P. C., and Harvey, A. R.
College/School:College of Science and Engineering > School of Physics and Astronomy
Journal Name:Optics Express
Publisher:Optical Society of America
ISSN:1094-4087
ISSN (Online):1094-4087
Published Online:25 July 2022
Copyright Holders:Copyright © The Author(s) 2022
First Published:First published in Optics Express 30(16): 29189-29205
Publisher Policy:Reproduced under a Creative Commons licence

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
190828EPSRC Centre for Doctoral Training in Sensing and MeasurementAndrew HarveyEngineering and Physical Sciences Research Council (EPSRC)EP/L016753/1P&S - Physics & Astronomy