TiO2 nanotopography-driven osteoblast adhesion through Coulomb’s force evolution

Luo, J. et al. (2022) TiO2 nanotopography-driven osteoblast adhesion through Coulomb’s force evolution. ACS Applied Materials and Interfaces, 14(30), pp. 34400-34414. (doi: 10.1021/acsami.2c07652) (PMID:35867934)

Abstract

Nanotopography is an effective method to regulate cells’ behaviors to improve Ti orthopaedic implants’ in vivo performance. However, the mechanism underlying cellular matrix–nanotopography interactions that allows the modulation of cell adhesion has remained elusive. In this study, we have developed novel nanotopographic features on Ti substrates and studied human osteoblast (HOb) adhesion on nanotopographies to reveal the interactive mechanism regulating cell adhesion and spreading. Through nanoflat, nanoconvex, and nanoconcave TiO2 nanotopographies, the evolution of Coulomb’s force between the extracellular matrix and nanotopographies has been estimated and comparatively analyzed, along with the assessment of cellular responses of HOb. We show that HObs exhibited greater adhesion and spreading on nanoconvex surfaces where they formed super matured focal adhesions and an ordered actin cytoskeleton. It also demonstrated that Coulomb’s force on nanoconvex features exhibits a more intense and concentrated evolution than that of nanoconcave features, which may result in a high dense distribution of fibronectin. Thus, this work is meaningful for novel Ti-based orthopaedic implants’ surface designs for enhancing their in vivo performance.

• Item Type: Articles
• Additional Information: This work was financially supported by the EU via the H2020- MSCA-RISE-2016 program (734156); Engineering and Physical Sciences Research Council via DTP CASE Programme (grant no: EP/T517793/1); and Royal Society via an International Exchange program (grant no: IEC/NSFC/191253).
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Salmeron-Sanchez, Professor Manuel and Luo, Mr Jiajun
• Authors: Luo, J., Zhao, S., Gao, X., Nath, S., Xu, W., Tamaddon, M., Thorogate, R., Yu, H., Lu, X., Salmeron-Sanchez, M., and Liu, C.
• College/School: College of Science and Engineering > School of Engineering > Biomedical Engineering
• Journal Name: ACS Applied Materials and Interfaces
• Publisher: American Chemical Society
• ISSN: 1944-8244
• ISSN (Online): 1944-8252
• Published Online: 22 July 2022
• Copyright Holders: Copyright © 2022 The Authors
• First Published: First published in ACS Applied Materials and Interfaces 14(30): 34400-34414
• Publisher Policy: Reproduced under a Creative Commons License