Spatiotemporal responses of trabecular and cortical bone to complete spinal cord injury in skeletally mature rats

Williams, J. A., Huesa, C., Windmill, J. F. C., Purcell, M., Reid, S., Coupaud, S. and Riddell, J. S. (2022) Spatiotemporal responses of trabecular and cortical bone to complete spinal cord injury in skeletally mature rats. Bone Reports, 16, 101592. (doi: 10.1016/j.bonr.2022.101592) (PMID:35637974) (PMCID:PMC9142855)

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Abstract

Objective: Characterise the spatiotemporal responses of trabecular and cortical bone to complete spinal cord injury (SCI) in the skeletally mature rat in the acute (4-week) period following injury. Methods: The spinal cord of 5-month old male rats was transected at the T9 level. Outcome measures were assessed using micro-computed tomography, three-point bending and serum markers at 1-, 2-, and 4-weeks post-transection. Comparison was made with time-0 and sham animals. Results: Lower levels of circulating serum bone formation markers and higher bone resorption markers suggested uncoupled bone turnover as early at 1-week post-transection. Micro-computed tomography showed metaphyseal and epiphyseal trabecular bone loss was observed only at 4-weeks post-transection. The bone loss was site-specific with a more severe reduction in trabecular BV/TV observed in the metaphyseal (50%) relative to epiphyseal (19%) region. Metaphyseal trabecular bone exhibited a 54% reduction in connectivity density while the epiphyseal trabecular bone was unaffected. Cortical bone deficits were not seen over the time periods examined. Conclusions: The study demonstrates that the skeletally mature spinal cord transected rat model replicates the biphasic pattern of osteoporotic changes observed in the human SCI population, providing a relevant model for testing the efficacy of interventions against SCI-induced osteoporosis.

Item Type:Articles
Additional Information:The study was supported by funding provided by the Science and Technology Facilities Council (STFC) Challenge Led Applied Systems Programme (ST/S000968/1) and (ST/S000852/1) and in part by the European Research Council under the European Union's Seventh Framework Programme (FP/2007-2013)/ERC Grant Agreement no. 615030. Carmen Huesa was supported by the Versus Arthritis early career fellowship (grant no. 22483).
Keywords:Osteoporosis, Mechanical testing, Skeletally mature, Spinal cord injury, Bone morphometry, microCT
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Huesa, Dr Carmen and Riddell, Professor John
Authors: Williams, J. A., Huesa, C., Windmill, J. F. C., Purcell, M., Reid, S., Coupaud, S., and Riddell, J. S.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
College of Medical Veterinary and Life Sciences > School of Psychology & Neuroscience
Journal Name:Bone Reports
Publisher:Elsevier
ISSN:2352-1872
ISSN (Online):2352-1872
Published Online:21 May 2022
Copyright Holders:Copyright © 2022 The Authors
First Published:First published in Bone Reports 16: 101592
Publisher Policy:Reproduced under a Creative Commons licence

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
302600Early diagnosis and intervention of osteoporosis using nanovibrational stimulationJohn RiddellScience and Technology Facilities Council (STFC)ST/S000852/1Centre for Neuroscience