Ijaz, S. et al. (2022) Mapping of SARS-CoV-2 IgM and IgG in gingival crevicular fluid: antibody dynamics and linkage to severity of COVID-19 in hospital inpatients. Journal of Infection, 85(2), pp. 152-160. (doi: 10.1016/j.jinf.2022.05.033) (PMID:35667482) (PMCID:PMC9163047)
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Abstract
Objectives: To characterise the acute and early convalescent antibody responses in gingival crevicular fluid (GCF) from patients hospitalised with COVID-19. Methods: IgM and IgG antibody against the SARS-CoV-2 Nucleoprotein (NP) and components of the Spike protein were analysed in GCF samples. The association between antibody reactivity and disease severity was also investigated. Results: IgM and IgG reactivity were detectable in GCF samples as early as week one post symptom onset. Differential patterns of antibody reactivity across the NP and Spike proteins were noted. Antibody responses to NP appeared earlier, a trend noted for both the IgM and IgG; however, the NP IgM response was not as robust as IgM responses to Spike proteins. There was some evidence that higher levels of antibody in GCF in the first 14 days post symptom onset were associated with a more severe disease and for this association to be more significant with the IgM response. Conclusions: Gingival crevicular fluid forms a useful analyte for the detection and monitoring of SARS-CoV-2 antibody responses. The data demonstrates a dynamic antibody response across different protein targets and indicates an association between severe disease and higher levels of antibody.
| Item Type: | Articles |
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| Additional Information: | This work is supported by grants from: the National Institute for Health Research (NIHR) [award CO-CIN-01], the Medical Research Council [grant MC_PC_19059] and by the NIHR Health Protection Research Unit (HPRU) in Emerging and Zoonotic Infections at University of Liverpool in partnership with UK Health Security Agency (UKHSA), in collaboration with Liverpool School of Tropical Medicine and the University of Oxford [award 200907], NIHR HPRU in Respiratory Infections at Imperial College London with UKHSA [award 200927], NIHR Biomedical Research Centre at Imperial College London [IS-BRC-1215-20013], and NIHR Clinical Research Network for providing infrastructure support for this research. The views expressed are those of the authors and not necessarily those of the NIHR, MRC or UKHSA. Funding was also available from UKRI CV220-11: Serological detection of past SARS-CoV-2 infection by non-invasive sampling for field epidemiology and quantitative antibody detection; held at Imperial College London. Work in PC’s laboratory was supported by the Francis Crick Institute, which receives its core funding from Cancer Research UK (FC001061), the Wellcome Trust (FC001061), and the UK Medical Research Council (FC001061). |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Ho, Dr Antonia and Vink, Elen |
| Authors: | Ijaz, S., Dicks, S., Jegatheesan, K., Parker, E., Katsanovskaja, K., Vink, E., McClure, M. O., Shute, J., Hope, J., Cook, N., Cherepanov, P., Turtle, L., Paxton, W. A., Pollakis, G., Ho, A., Openshaw, P. J.M., Baillie, J. K., Semple, M. G., and Tedder, R. S. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Journal Name: | Journal of Infection |
| Publisher: | Elsevier |
| ISSN: | 0163-4453 |
| ISSN (Online): | 1532-2742 |
| Published Online: | 03 June 2022 |
| Copyright Holders: | Copyright © 2022 Crown Copyright |
| First Published: | First published in Journal of Infection 85(2): 152-160 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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