SUMOylation does not affect cardiac troponin I stability but alters indirectly the development of force in response to Ca2+

Fertig, B. et al. (2022) SUMOylation does not affect cardiac troponin I stability but alters indirectly the development of force in response to Ca2+. FEBS Journal, (doi: 10.1111/febs.16537) (PMID:35633070) (Early Online Publication)

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Abstract

Post-translational modification of the myofilament protein troponin I by phosphorylation is known to trigger functional changes that support enhanced contraction and relaxation of the heart. We report for the first time that human troponin I can also be modified by SUMOylation at lysine 177. Functionally, TnI SUMOylation is not a factor in the development of passive and maximal force generation in response to calcium, however this modification seems to act indirectly by preventing SUMOylation of other myofilament proteins to alter calcium sensitivity and cooperativity of myofilaments. Utilising a novel, custom SUMO site-specific antibody that recognises only the SUMOylated form of troponin I, we verify that this modification occurs in human heart and that it is upregulated during disease.

Item Type:Articles
Additional Information:This work was funded by the BHF PhD studentship programme U.K. to B.F. and G.S.B. BHF Grant FS/15/64/32035. Funding: American Heart Association TP135689, NIH HL148785, University of Kentucky Myocardial Recovery Alliance.
Status:Early Online Publication
Refereed:Yes
Glasgow Author(s) Enlighten ID:Dobi, Sara and Smith, Professor Godfrey and Baillie, Professor George and Tibbo, Amy and LING, JIAYUE and Fertig, Bracy and Busiau, Tara
Authors: Fertig, B., Ling, J., Nollet, E.E., Dobi, S., Busiau, T., Ishikawa, K., Yamada, K., Lee, A., Kho, C., Wills, L., Tibbo, A., Scott, M., Grant, K., Campbell, K.S., Birks, E.J., MacQuaide, N., Hajjar, R., Smith, G.L., van der Velden, J., and Baillie, G.
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
Journal Name:FEBS Journal
Publisher:Wiley
ISSN:1742-464X
ISSN (Online):1742-4658
Published Online:27 May 2022
Copyright Holders:Copyright © 2022 The Authors
First Published:First published in FEBS Journal 2022
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
172199BHF 4 Year PhD Studentship Award (3rd intake 2015)Rhian TouyzBritish Heart Foundation (BHF)FS/15/64/32035Institute of Cardiovascular & Medical Sciences