Evaluation of the specificity of five oligoprobes for identification of cyathostomin species from horses

Hodgkinson, J., Love, S., Lichtenfels, J., Palfreman, S., Ramsey, Y. and Matthews, J. (2001) Evaluation of the specificity of five oligoprobes for identification of cyathostomin species from horses. International Journal for Parasitology, 31(2), pp. 197-204. (doi:0.1016/S0020-7519(00)00161-2)

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Abstract

Here, we report evaluation of five oligoprobes designed from intergenic spacer (IGS) region sequences for identification of cyathostomin species. Oligoprobes were designed for identification of Cylicocyclus ashworthi, Cylicocyclus nassatus, Cylicostephanus longibursatus, Cylicostephanus goldi and a fifth probe designed to identify all members of this tribe. PCR amplification of IGS DNA from 16 cyathostomin species allowed sequence comparison and identification of four putative species-specific probes. Southern blotting of amplified products from 16 species showed that all probes were species-specific. The fifth probe recognised all 16 cyathostomin species but did not bind to members of the genus Strongylus. Furthermore, these probes were used to identify individual infective L3, eggs and L4 indicating that they will be invaluable to furthering the study of the epidemiology and pathogenesis of these important equine nematodes.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Love, Professor Sandy
Authors: Hodgkinson, J., Love, S., Lichtenfels, J., Palfreman, S., Ramsey, Y., and Matthews, J.
College/School:College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:International Journal for Parasitology
Publisher:Elsevier
ISSN:0020-7519
ISSN (Online):1879-0135
Published Online:28 February 2001

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