Protein-coated nanostructured surfaces affect the adhesion of Escherichia coli

Kallas, P., Valen, H., Hulander, M., Gadegaard, N. , Stormonth-Darling, J. , O'Reilly, P., Thiede, B., Andersson, M. and Haugen, H. J. (2022) Protein-coated nanostructured surfaces affect the adhesion of Escherichia coli. Nanoscale, 14(20), pp. 7736-7746. (doi: 10.1039/D2NR00976E) (PMID:35579413) (PMCID:PMC9135173)

Abstract

Developing new implant surfaces with anti-adhesion bacterial properties used for medical devices remains a challenge. Here we describe a novel study investigating nanotopography influences on bacterial adhesion on surfaces with controlled interspatial nanopillar distances. The surfaces were coated with proteins (fibrinogen, collagen, serum and saliva) prior to E. coli-WT adhesion under flow conditions. PiFM provided chemical mapping and showed that proteins adsorbed both between and onto the nanopillars with a preference for areas between the nanopillars. E. coli-WT adhered least to protein-coated areas with low surface nanopillar coverage, most to surfaces coated with saliva, while human serum led to the lowest adhesion. Protein-coated nanostructured surfaces affected the adhesion of E. coli-WT.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Stormonth-Darling, Mr John and Gadegaard, Professor Nikolaj

Creator Roles

Gadegaard, N.: Conceptualization, Methodology
Stormonth-Darling, J.: Data curation, Investigation

• Authors: Kallas, P., Valen, H., Hulander, M., Gadegaard, N., Stormonth-Darling, J., O'Reilly, P., Thiede, B., Andersson, M., and Haugen, H. J.
• College/School: College of Science and Engineering > School of Engineering > Biomedical Engineering
• Journal Name: Nanoscale
• Publisher: Royal Society of Chemistry
• ISSN: 2040-3364
• ISSN (Online): 2040-3372
• Published Online: 28 April 2022
• Copyright Holders: Copyright © 2022 The Royal Society of Chemistry
• First Published: First published in Nanoscale 14(20): 7736-7746
• Publisher Policy: Reproduced under a Creative Commons License

Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
172025		FAKIR: Focal Adhesion Kinetics In nanosurface Recognition	Nikolaj Gadegaard	European Research Council (ERC)	648892	ENG - Biomedical Engineering