Myelinated axons are the primary target of hemin-mediated oxidative damage in a model of the central nervous system

Baldacchino, K. et al. (2022) Myelinated axons are the primary target of hemin-mediated oxidative damage in a model of the central nervous system. Experimental Neurology, 354, 114113. (doi: 10.1016/j.expneurol.2022.114113) (PMID:35569511)

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Abstract

Iron released from oligodendrocytes during demyelination or derived from haemoglobin breakdown products is believed to amplify oxidative tissue injury in multiple sclerosis (MS). However, the pathophysiological significance of iron-containing haemoglobin breakdown products themselves is rarely considered in the context of MS and their cellular specificity and mode of action remain unclear. Using myelinating cell cultures, we now report the cytotoxic potential of hemin (ferriprotoporphyrin IX chloride), a major degradation product of haemoglobin, is 25-fold greater than equimolar concentrations of free iron in myelinating cultures; a model that reproduces the complex multicellular environment of the CNS. At low micro molar concentrations (3.3 - 10 μM) we observed hemin preferentially binds to myelin and axons to initiate a complex detrimental response that results in targeted demyelination and axonal loss but spares neuronal cell bodies, astrocytes and the majority of oligodendroglia. Demyelination and axonal loss in this context are executed by a combination of mechanisms that include iron-dependent peroxidation by reactive oxygen species (ROS) and ferroptosis. These effects are microglial-independent, do not require any initiating inflammatory insult and represent a direct effect that compromises the structural integrity of myelinated axons in the CNS. Our data identify hemin-mediated demyelination and axonal loss as a novel mechanism by which intracerebral degradation of haemoglobin may contribute to lesion development in MS.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Muecklisch, Dr Katja and Barnett, Professor Susan and Edgar, Professor Julia and Komarek, Miss Lina and Peveler, Dr William and Baldacchino, Mr Karl and Lindsay, Dr Susan and Linington, Professor Christopher and Hayden, Lorna and Lemgruber Soares, Dr Leandro and Sherrard Smith, rebecca
Authors: Baldacchino, K., Peveler, W. J., Lemgruber, L., Sherrard Smith, R., Scharler, C., Hayden, L., Komarek, L., Lindsay, S. L., Barnett, S. C., Edgar, J. M., Linington, C., and Thümmler, K.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
College of Science and Engineering > School of Chemistry
Journal Name:Experimental Neurology
Publisher:Elsevier
ISSN:0014-4886
ISSN (Online):1090-2430
Published Online:13 May 2022
Copyright Holders:Copyright © 2022 The Authors
First Published:First published in Experimental Neurology 354: 114113
Publisher Policy:Reproduced under a Creative Commons License

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