de Vito, G. et al. (2022) Fast whole-brain imaging of seizures in zebrafish larvae by two-photon light-sheet microscopy. Biomedical Optics Express, 13(3), pp. 1516-1536. (doi: 10.1364/BOE.434146) (PMID:35414999) (PMCID:PMC8973167)
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Abstract
Light-sheet fluorescence microscopy (LSFM) enables real-time whole-brain functional imaging in zebrafish larvae. Conventional one-photon LSFM can however induce undesirable visual stimulation due to the use of visible excitation light. The use of two-photon (2P) excitation, employing near-infrared invisible light, provides unbiased investigation of neuronal circuit dynamics. However, due to the low efficiency of the 2P absorption process, the imaging speed of this technique is typically limited by the signal-to-noise-ratio. Here, we describe a 2P LSFM setup designed for non-invasive imaging that enables quintuplicating state-of-the-art volumetric acquisition rate of the larval zebrafish brain (5 Hz) while keeping low the laser intensity on the specimen. We applied our system to the study of pharmacologically-induced acute seizures, characterizing the spatial-temporal dynamics of pathological activity and describing for the first time the appearance of caudo-rostral ictal waves (CRIWs).
Item Type: | Articles |
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Additional Information: | Funding: European Research Council (692943); Ministero dell’Istruzione, dell’Università e della Ricerca (Eurobioimaging Italian Nodes); Fondazione Cassa di Risparmio di Firenze (2020.1664 and 2020.1666). |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Muellenbroich, Dr Caroline |
Authors: | de Vito, G., Turrini, L., Müllenbroich, C., Ricci, P., Sancataldo, G., Mazzamuto, G., Tiso, N., Sacconi, L., Fanelli, D., Silvestri, L., Vanzi, F., and Pavone, F. S. |
College/School: | College of Science and Engineering > School of Physics and Astronomy |
Journal Name: | Biomedical Optics Express |
Publisher: | Optical Society of America |
ISSN: | 2156-7085 |
ISSN (Online): | 2156-7085 |
Published Online: | 16 February 2022 |
Copyright Holders: | Copyright © 2022 Optical Society of America |
First Published: | First published in Biomedical Optics Express 13(3): 1516-1536 |
Publisher Policy: | Reproduced in accordance with the publisher copyright policy |
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