pereira, P. M., Jacobs, C. and Grove, J. (2018) Investigating hepatitis C virus infection using super-resolution microscopy. In: Law, M. (ed.) Hepatitis C Virus Protocols. Series: Methods in molecular biology (1911). Humana Press: New York, NY, pp. 247-261. ISBN 9781493989751 (doi: 10.1007/978-1-4939-8976-8_17)
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Abstract
Super-resolution microscopy (SRM) can provide a window on the nanoscale events of virus replication. Here we describe a protocol for imaging hepatitis C virus-infected cells using localization SRM. We provide details on sample preparation, immunostaining, data collection, and super-resolution image reconstruction. We have made all efforts to generalize the protocol to make it accessible to all budding super-resolution microscopists.
| Item Type: | Book Sections |
|---|---|
| Status: | Published |
| Glasgow Author(s) Enlighten ID: | Grove, Dr Joe |
| Authors: | pereira, P. M., Jacobs, C., and Grove, J. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Publisher: | Humana Press |
| ISBN: | 9781493989751 |
| Published Online: | 29 December 2018 |
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