Deploying an in vitro gut model to assay the impact of the mannan-oligosaccharide prebiotic bio-mos on the Atlantic Salmon (Salmo salar) gut microbiome

Kazlauskaite, R. et al. (2022) Deploying an in vitro gut model to assay the impact of the mannan-oligosaccharide prebiotic bio-mos on the Atlantic Salmon (Salmo salar) gut microbiome. Microbiology Spectrum, 10(3), e0195321. (doi: 10.1128/spectrum.01953-21) (PMID:35532227) (PMCID:PMC9241627)

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Abstract

Alpha mannose-oligosaccharide (MOS) prebiotics are widely deployed in animal agriculture as immunomodulators as well as to enhance growth and gut health. Their mode of action is thought to be mediated through their impact on host microbial communities and their associated metabolism. Bio-Mos is a commercially available prebiotic currently used in the agri-feed industry, but studies show contrasting results of its effect on fish performance and feed efficiency. Thus, detailed studies are needed to investigate the effect of MOS supplements on the fish microbiome to enhance our understanding of the link between MOS and gut health. To assess Bio-Mos for potential use as a prebiotic growth promoter in salmonid aquaculture, we have modified an established Atlantic salmon in vitro gut model, SalmoSim, to evaluate its impact on the host microbial communities. The microbial communities obtained from ceca compartments from four adult farmed salmon were inoculated in biological triplicate reactors in SalmoSim. Prebiotic treatment was supplemented for 20 days, followed by a 6-day washout period. Inclusion of Bio-Mos in the media resulted in a significant increase in formate (P = 0.001), propionate (P = 0.037) and 3-methyl butanoic acid (P = 0.024) levels, correlated with increased abundances of several, principally, anaerobic microbial genera (Fusobacterium, Agarivorans, Pseudoalteromonas). DNA metabarcoding with the 16S rDNA marker confirmed a significant shift in microbial community composition in response to Bio-Mos supplementation with observed increase in lactic acid producing Carnobacterium. In conjunction with previous in vivo studies linking enhanced volatile fatty acid production alongside MOS supplementation to host growth and performance, our data suggest that Bio-Mos may be of value in salmonid production. Furthermore, our data highlights the potential role of in vitro gut models to complementin vivo trials of microbiome modulators.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Heys, Dr Chloe and Kazlauskaite, Raminta and Llewellyn, Professor Martin and Cheaib, Dr Bachar and Sloan, Professor William and Russell, Mrs Julie and Humble, Joseph and Ijaz, Dr Umer and Connelly, Dr Stephanie
Authors: Kazlauskaite, R., Cheaib, B., Humble, J., Heys, C., Ijaz, U. Z., Connelly, S., Sloan, W. T., Russell, J., Martinez-Rubio, L., Sweetman, J., Kitts, A., McGinnity, P., Lyons, P., and Llewellyn, M. S.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
College of Science and Engineering > School of Engineering > Infrastructure and Environment
College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
Journal Name:Microbiology Spectrum
Publisher:American Society for Microbiology
ISSN:2165-0497
ISSN (Online):2165-0497
Published Online:09 May 2022
Copyright Holders:Copyright © 2022 Kazlauskaite et al.
First Published:First published in Microbiology Spectrum 10(3): e0195321
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
173483A microbial basis for Atlantic Salmon energeticsMartin LlewellynBiotechnology and Biological Sciences Research Council (BBSRC)BB/P001203/1LS - Animal Biology
173445RCUK-CONICYT Mucosal health and microbiota during sea lice parasitism: effect of oral delivery of immunomodulantsMartin LlewellynBiotechnology and Biological Sciences Research Council (BBSRC)BB/N024028/1LS - Animal Biology
170256Understanding microbial community through in situ environmental 'omic data synthesisUmer Zeeshan IjazNatural Environment Research Council (NERC)NE/L011956/1ENG - Infrastructure & Environment