The effect of the polymerized bovine haemoglobin solution, Hb-200, on endothelial function in isolated arterial rings from rats

Pawson, P., Gibson, I.F. and Dowell, F.J. (2007) The effect of the polymerized bovine haemoglobin solution, Hb-200, on endothelial function in isolated arterial rings from rats. Journal of Veterinary Pharmacology and Therapeutics, 30(6), pp. 556-563. (doi:10.1111/j.1365-2885.2007.00913.x)

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Abstract

Haemoglobin-based oxygen carriers have been developed as ‘blood substitutes’. Early cross-linked haemoglobins caused marked vasoconstriction, however, polymerized haemoglobin solutions, such as the bovine product haemoglobin glutamer-200 (bovine) (Hb-200), are said to have lesser vascular effects. The aim of this study was to quantify the effect of Hb-200 on isolated rat arterial rings. The actions of Hb-200 were investigated using rings of aorta and second-order mesenteric arteries from female Wistar rats. The arterial rings were mounted for isometric tension recording. Addition of Hb-200 (1 μm) to arterial rings, precontracted with phenylephrine (50–70% maximum phenylephrine-induced tone), resulted in vasoconstriction. Addition of Hb-200 to arteries precontracted with phenylephrine and relaxed with acetylcholine (1 μm) also caused contraction. Furthermore preincubation with Hb-200 (1 μm) enhanced the response to phenylephrine (1 nm to 10 μm) and impaired acetylcholine-induced relaxation (10 nm to 10 μm). Responses to Hb-200 were similar to those in response to the nitric oxide (NO) synthase inhibitor, NG-nitro-l-arginine methyl ester (l-NAME, 100 μm), alone or in combination with Hb-200. These data demonstrate that Hb-200 has a significant vasoconstrictor effect similar to l-NAME. Thus the vascular actions of Hb-200 are consistent with activity as a NO scavenger. This may have implications for the clinical use of Hb-200.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Pawson, Dr Patricia and Dowell, Dr Fiona and Gibson, Mr Ian
Authors: Pawson, P., Gibson, I.F., and Dowell, F.J.
College/School:College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:Journal of Veterinary Pharmacology and Therapeutics
ISSN:0140-7783
Published Online:24 October 2007

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