Nguyen, T. X. et al. (2022) Abundance of plasma proteins in response to divergent ratios of dietary ω6:ω3 fatty acids in gestating and lactating sows using a quantitative proteomics approach. Journal of Proteomics, 260, 104562. (doi: 10.1016/j.jprot.2022.104562) (PMID:35314360)
![[img]](https://eprints.gla.ac.uk/style/images/fileicons/text.png) |
Text
268581.pdf - Published Version Available under License Creative Commons Attribution. 2MB |
Abstract
This study aimed to investigate the characteristic proteomic pattern of plasma from sows supplemented with low dietary ω6:ω3 fatty acids (FAs) ratio during gestation and lactation. Two dietary treatments (n = 8 each) comprised either a control ratio of ω6:ω3 FAs (CR, 13:1 during gestation and 10:1 during lactation) or a low ratio (LR, 4:1 during gestation and lactation) by adding soybean oil or linseed oil, respectively. High-resolution mass spectrometry-based quantitative proteomics was applied on plasma (n = 5 each) at day 108 of gestation (G108) and at the end of lactation (L-End), and a total of 379 proteins and 202 master proteins were identified. Out of these, four differentially abundant proteins between LR and CR samples at G108 may relate to serine-type endopeptidase inhibitor activity. Differentially abundant proteins in L-End versus G108 (12 up-regulated and 10 down-regulated) were positively correlated with the events that regulate plasma lipoproteins, stimulus- and defence-responses. These findings demonstrate the benefit of increased dietary ω3 FAs in modifying proteins involved in protective mechanisms against increased stresses in key life cycle phases in pigs. In addition, proteome changes from late gestation to late lactation disclosed the underlying mechanism of pigs in response to reproduction-related stimuli.
| Item Type: | Articles |
|---|---|
| Additional Information: | This project is supported by the European Unions' Horizon 2020 research and innovation programme H2020-MSCA-ITN-2017-EJD under the Marie Skłodowska-Curie Innovative Training Networks (European Joint Doctorate in Molecular Animal Nutrition, MANNA), grant agreement No 765423. |
| Keywords: | Haptoglobin, High-density lipoprotein, Gestation, Lactation, Plasma proteome, ω6:ω3 fatty acids |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | McLaughlin, Dr Mark and McGill, Mrs Suzanne and Burchmore, Dr Richard and Weidt, Dr Stefan and Nguyen, Thi Xuan |
| Authors: | Nguyen, T. X., Agazzi, A., McGill, S., Weidt, S., Han, Q. H., Gelemanović, A., McLaughlin, M., Piñeiro, M., Savoini, G., Eckersall, P. D., and Burchmore, R. |
| College/School: | College of Medical Veterinary and Life Sciences College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Journal of Proteomics |
| Publisher: | Elsevier |
| ISSN: | 1874-3919 |
| ISSN (Online): | 1876-7737 |
| Published Online: | 18 March 2022 |
| Copyright Holders: | Copyright © 2022 The Authors |
| First Published: | First published in Journal of Proteomics 260:104562 |
| Publisher Policy: | Reproduced under a Creative Commons License |
University Staff: Request a correction | Enlighten Editors: Update this record
Funder and Project Information
Funder and Project Information