Single-cell transcriptomics reveals expression profiles of Trypanosoma brucei sexual stages

Howick, V. M. , Peacock, L., Kay, C., Collett, C., Gibson, W. and Lawniczak, M. K.N. (2022) Single-cell transcriptomics reveals expression profiles of Trypanosoma brucei sexual stages. PLoS Pathogens, 18(3), e1010346. (doi: 10.1371/journal.ppat.1010346) (PMID:35255094) (PMCID:PMC8939820)

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Abstract

Early diverging lineages such as trypanosomes can provide clues to the evolution of sexual reproduction in eukaryotes. In Trypanosoma brucei, the pathogen that causes Human African Trypanosomiasis, sexual reproduction occurs in the salivary glands of the insect host, but analysis of the molecular signatures that define these sexual forms is complicated because they mingle with more numerous, mitotically-dividing developmental stages. We used single-cell RNA-sequencing (scRNAseq) to profile 388 individual trypanosomes from midgut, proventriculus, and salivary glands of infected tsetse flies allowing us to identify tissue-specific cell types. Further investigation of salivary gland parasite transcriptomes revealed fine-scale changes in gene expression over a developmental progression from putative sexual forms through metacyclics expressing variant surface glycoprotein genes. The cluster of cells potentially containing sexual forms was characterized by high level transcription of the gamete fusion protein HAP2, together with an array of surface proteins and several genes of unknown function. We linked these expression patterns to distinct morphological forms using immunofluorescence assays and reporter gene expression to demonstrate that the kinetoplastid-conserved gene Tb927.10.12080 is exclusively expressed at high levels by meiotic intermediates and gametes. Further experiments are required to establish whether this protein, currently of unknown function, plays a role in gamete formation and/or fusion.

Item Type:Articles
Additional Information:This research was supported by BBSRC Grants BB/R016437/1 and BB/R010188/1 to WG, Wellcome Trust Grant 206194/Z/17/Z to the Wellcome Sanger Institute, and a Sir Henry Dale Fellowship jointly funded by the Wellcome Trust and the Royal Society (Grant 220185/Z/20/Z) to VMH. LP, CK and CC received salary from BBSRC. VMH and MKNL received salary from Wellcome Trust.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Howick, Dr Virginia
Creator Roles:
Howick, V. M.Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Visualization, Writing – original draft, Writing – review and editing
Authors: Howick, V. M., Peacock, L., Kay, C., Collett, C., Gibson, W., and Lawniczak, M. K.N.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
Journal Name:PLoS Pathogens
Publisher:Public Library of Science
ISSN:1553-7366
ISSN (Online):1553-7374
Published Online:07 March 2022
Copyright Holders:Copyright © 2022 Howick et al.
First Published:First published in PLoS Pathogens 18(3): e1010346
Publisher Policy:Reproduced under a Creative Commons License
Data DOI:10.5281/zenodo.6047732

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
308631Identification of genomic components that predict transmission of the malaria parasite in different vector speciesVirginia HowickWellcome Trust (WELLCOTR)220185/Z/20/ZMVLS - Polyomics Facility