Fly Cell Atlas: A single-nucleus transcriptomic atlas of the adult fruit fly

Li, H. et al. (2022) Fly Cell Atlas: A single-nucleus transcriptomic atlas of the adult fruit fly. Science, 375(6584), eabk2432. (doi: 10.1126/science.abk2432) (PMID:35239393) (PMCID:PMC8944923)

Abstract

For more than 100 years, the fruit fly Drosophila melanogaster has been one of the most studied model organisms. Here, we present a single-cell atlas of the adult fly, Tabula Drosophilae , that includes 580,000 nuclei from 15 individually dissected sexed tissues as well as the entire head and body, annotated to >250 distinct cell types. We provide an in-depth analysis of cell type–related gene signatures and transcription factor markers, as well as sexual dimorphism, across the whole animal. Analysis of common cell types between tissues, such as blood and muscle cells, reveals rare cell types and tissue-specific subtypes. This atlas provides a valuable resource for the Drosophila community and serves as a reference to study genetic perturbations and disease models at single-cell resolution.

• Item Type: Articles
• Additional Information: Funding: The sequencing was supported by the Chan Zuckerberg Biohub (S.R.Q.), Genentech Inc. (H.J.), National Institutes of Health intramural 1ZIADK015600 (B.O.), national funds through the FCT in the framework of the financing of the Norma Transitória DL 57/2016 (Z.C.-S.), Wu Tsai Neurosciences Institute at Stanford (S.R.Q. and L.L.), and the Howard Hughes Medical Institute and a National Institutes of Health grant (L.L.). Computational work was supported by the KU Leuven and the Flemish Supercomputer Center (VSC) (S.A.) and EPFL (B.D.). FCA Consortium funding is provided in the supplementary materials.
• Keywords: Multidisciplinary
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Dow, Professor Julian
• Authors: Li, H., Janssens, J., De Waegeneer, M., Kolluru, S. S., Davie, K., Gardeux, V., Saelens, W., David, F. P. A., Brbić, M., Spanier, K., Leskovec, J., McLaughlin, C. N., Xie, Q., Jones, R. C., Brueckner, K., Shim, J., Tattikota, S. G., Schnorrer, F., Rust, K., Nystul, T. G., Carvalho-Santos, Z., Ribeiro, C., Pal, S., Mahadevaraju, S., Przytycka, T. M., Allen, A. M., Goodwin, S. F., Berry, C. W., Fuller, M. T., White-Cooper, H., Matunis, E. L., DiNardo, S., Galenza, A., O’Brien, L. E., Dow, J. A. T., Jasper, H., Oliver, B., Perrimon, N., Deplancke, B., Quake, S. R., Luo, L., Aerts, S., Agarwal, D., Ahmed-Braimah, Y., Arbeitman, M., Ariss, M. M., Augsburger, J., Ayush, K., Baker, C. C., Banisch, T., Birker, K., Bodmer, R., Bolival, B., Brantley, S. E., Brill, J. A., Brown, N. C., Buehner, N. A., Cai, X. T., Cardoso-Figueiredo, R., Casares, F., Chang, A., Clandinin, T. R., Crasta, S., Desplan, C., Detweiler, A. M., Dhakan, D. B., Donà, E., Engert, S., Floc’hlay, S., George, N., González-Segarra, A. J., Groves, A. K., Gumbin, S., Guo, Y., Harris, D. E., Heifetz, Y., Holtz, S. L., Horns, F., Hudry, B., Hung, R.-J., Jan, Y. N., Jaszczak, J. S., Jefferis, G. S. X. E., Karkanias, J., Karr, T. L., Katheder, N. S., Kezos, J., Kim, A. A., Kim, S. K., Kockel, L., Konstantinides, N., Kornberg, T. B., Krause, H. M., Labott, A. T., Laturney, M., Lehmann, R., Leinwand, S., Li, J., Li, J. S. S., Li, K., Li, K., Li, L., Li, T., Litovchenko, M., Liu, H.-H., Liu, Y., Lu, T.-C., Manning, J., Mase, A., Matera-Vatnick, M., Matias, N. R., McDonough-Goldstein, C. E., McGeever, A., McLachlan, A. D., Moreno-Roman, P., Neff, N., Neville, M., Ngo, S., Nielsen, T., O’Brien, C. E., Osumi-Sutherland, D., Özel, M. N., Papatheodorou, I., Petkovic, M., Pilgrim, C., Pisco, A. O., Reisenman, C., Sanders, E. N., dos Santos, G., Scott, K., Sherlekar, A., Shiu, P., Sims, D., Sit, R. V., Slaidina, M., Smith, H. E., Sterne, G., Su, Y.-H., Sutton, D., Tamayo, M., Tan, M., Tastekin, I., Treiber, C., Vacek, D., Vogler, G., Waddell, S., Wang, W., Wilson, R. I., Wolfner, M. F., Wong, Y.-C. E., Xie, A., Xu, J., Yamamoto, S., Yan, J., Yao, Z., Yoda, K., Zhu, R., and Zinzen, R. P.
• College/School: College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
• Journal Name: Science
• Publisher: American Association for the Advancement of Science
• ISSN: 0036-8075
• ISSN (Online): 1095-9203