Binding of more than one Tva800 molecule is required for ASLV-A entry

Gray, E. R., Illingworth, C. J.R. , Coffin, J. M. and Stoye, J. P. (2011) Binding of more than one Tva800 molecule is required for ASLV-A entry. Retrovirology, 8(1), 96. (doi: 10.1186/1742-4690-8-96) (PMID:22099981) (PMCID:PMC3267798)

Abstract

Background: Understanding the mechanism by which viruses enter their target cell is an essential part of understanding their infectious cycle. Previous studies have focussed on the multiplicity of viral envelope proteins that need to bind to their cognate receptor to initiate entry. Avian sarcoma and leukosis virus Envelope protein (ASLV Env) mediates entry via a receptor, Tva, which can be attached to the cell surface either by a phospholipid anchor (Tva800) or a transmembrane domain (Tva950). In these studies, we have now investigated the number of target receptors necessary for entry of ASLV Env-pseudotyped virions. Results: Using titration and modelling experiments we provide evidence that binding of more than one receptor, probably two, is needed for entry of virions via Tva800. However, binding of just one Tva950 receptor is sufficient for successful entry. Conclusions: The different modes of attachment of Tva800 and Tva950 to the cell membrane have important implications for the utilisation of these proteins as receptors for viral binding and/or uptake.

• Item Type: Articles
• Additional Information: This work was supported by the UK Medical Research Council file reference U117512710 (JPS) and by NIH research grant R37 CA 089441 (JMC). JMC was a Research Professor of the American Cancer Society with support from the F.M. Kirby Foundation. CJRI was supported in part by the Wellcome Trust under grant reference 091747.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Illingworth, Dr Chris
• Authors: Gray, E. R., Illingworth, C. J.R., Coffin, J. M., and Stoye, J. P.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity; College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
• Journal Name: Retrovirology
• Publisher: BioMed Central
• ISSN: 1742-4690
• ISSN (Online): 1742-4690
• Copyright Holders: Copyright © 2011 The Authors
• First Published: First published in Retrovirology 8(1):96
• Publisher Policy: Reproduced under a Creative Commons License