Mapping the influence of the gut microbiota on small molecules across the microbiome gut brain axis

Hulme, H. et al. (2022) Mapping the influence of the gut microbiota on small molecules across the microbiome gut brain axis. Journal of the American Society for Mass Spectrometry, 33(4), pp. 649-659. (doi: 10.1021/jasms.1c00298) (PMID:35262356)

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Abstract

Microbes exert influence across the microbiome–gut–brain axis through neurotransmitter production, induction of host immunomodulators, or the release or induction of other microbial or host molecules. Here, we used mass spectrometry imaging (MSI), a label-free imaging tool, to map molecular changes in the gut and brain in germ-free, antibiotic-treated and control mice. We determined spatial distribution and relative quantification of neurotransmitters and their precursors in response to the microbiome. Using untargeted MSI, we detected a significant change in the levels of four identified small molecules in the brains of germ-free animals compared to controls. However, antibiotic treatment induced no significant changes in these same metabolites in the brain after 1 week of treatment. This work exemplifies the utility of MSI as a tool for the study of known and discovery of novel, mediators of microbiome-gut-brain axis communication.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:HULME, Heather and Burchmore, Dr Richard and Wall, Dr Daniel and Milling, Professor Simon and Meikle, Dr Lynsey
Authors: Hulme, H., Meikle, L. M., Strittmatter, N., Swales, J., Hamm, G., Brown, S. L., Milling, S., MacDonald, A. S., Goodwin, R. J. A., Burchmore, R., and Wall, D. M.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:Journal of the American Society for Mass Spectrometry
Publisher:American Chemical Society
ISSN:1044-0305
ISSN (Online):1879-1123
Published Online:09 March 2022
Copyright Holders:Copyright © 2022 American Society for Mass Spectrometry.
First Published:First published in Journal of the American Society for Mass Spectrometry 33(4): 649-659
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
167488Survival and dissemination of enteric pathogens through exploitation and inhibition of programmed cell death pathways in circulating immune cells.Daniel WallBiotechnology and Biological Sciences Research Council (BBSRC)BB/K008005/1III - Bacteriology
173261Propionic acid use in agriculture and food production is driving evolution of novel Escherichia coli pathotypesDaniel WallBiotechnology and Biological Sciences Research Council (BBSRC)BB/P003281/1III - Bacteriology