Transcriptomal analysis of varicella-zoster virus infection using long oligonucleotide-based microarrays

Kennedy, P.G.E., Grinfeld, E., Craigon, M., Vierlinger, K., Roy, D., Forster, T. and Ghazal, P. (2005) Transcriptomal analysis of varicella-zoster virus infection using long oligonucleotide-based microarrays. Journal of General Virology, 86(10), pp. 2673-2684. (doi: 10.1099/vir.0.80946-0)

Full text not currently available from Enlighten.

Abstract

Varicella-zoster virus (VZV) is a human herpes virus that causes varicella as a primary infection and herpes zoster following reactivation of the virus from a latent state in trigeminal and spinal ganglia. In order to study the global pattern of VZV gene transcription, VZV microarrays using 75-base oligomers to 71 VZV open reading frames (ORFs) were designed and validated. The long-oligonucleotide approach maximizes the stringency of detection and polarity of gene expression. To optimize sensitivity, microarrays were hybridized to target RNA and the extent of hybridization measured using resonance light scattering. Microarray data were normalized to a subset of invariant ranked host-encoded positive-control genes and the data subjected to robust formal statistical analysis. The programme of viral gene expression was determined for VZV (Dumas strain)-infected MeWo cells and SVG cells (an immortalized human astrocyte cell line) 72 h post-infection. Marked quantitative and qualitative differences in the viral transcriptome were observed between the two different cell types using the Dumas laboratory-adapted strain. Oligonucleotide-based VZV arrays have considerable promise as a valuable tool in the analysis of viral gene transcription during both lytic and latent infections, and the observed heterogeneity in the global pattern of viral gene transcription may also have diagnostic potential.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Kennedy, Professor Peter and Grinfeld, Mrs Esther
Authors: Kennedy, P.G.E., Grinfeld, E., Craigon, M., Vierlinger, K., Roy, D., Forster, T., and Ghazal, P.
Subjects:R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of General Virology
Journal Abbr.:J. Gen. Virol.
Publisher:Society for General Microbiology
ISSN:0022-1317
ISSN (Online):1465-2099

University Staff: Request a correction | Enlighten Editors: Update this record