Host tissue proteomics reveal insights into the molecular basis of Schistosoma haematobium-induced bladder pathology

Osakunor, D. N.M., Ishida, K., Lamanna, O. K., Rossi, M., Dwomoh, L. and Hsieh, M. H. (2022) Host tissue proteomics reveal insights into the molecular basis of Schistosoma haematobium-induced bladder pathology. PLoS Neglected Tropical Diseases, 16(2), e0010176. (doi: 10.1371/journal.pntd.0010176) (PMID:35167594) (PMCID:PMC8846513)

Abstract

Article Authors Metrics Comments Media Coverage Abstract Author summary Introduction Methods Results Discussion Conclusion Supporting information Acknowledgments References Reader Comments Figures Abstract Urogenital schistosomiasis remains a major public health concern worldwide. In response to egg deposition, the host bladder undergoes gross and molecular morphological changes relevant for disease manifestation. However, limited mechanistic studies to date imply that the molecular mechanisms underlying pathology are not well-defined. We leveraged a mouse model of urogenital schistosomiasis to perform for the first time, proteome profiling of the early molecular events that occur in the bladder after exposure to S. haematobium eggs, and to elucidate the protein pathways involved in urogenital schistosomiasis-induced pathology. Purified S. haematobium eggs or control vehicle were microinjected into the bladder walls of mice. Mice were sacrificed seven days post-injection and bladder proteins isolated and processed for proteome profiling using mass spectrometry. We demonstrate that biological processes including carcinogenesis, immune and inflammatory responses, increased protein translation or turnover, oxidative stress responses, reduced cell adhesion and epithelial barrier integrity, and increased glucose metabolism were significantly enriched in S. haematobium infection. S. haematobium egg deposition in the bladder results in significant changes in proteins and pathways that play a role in pathology. Our findings highlight the potential bladder protein indicators for host-parasite interplay and provide new insights into the complex dynamics of pathology and characteristic bladder tissue changes in urogenital schistosomiasis. The findings will be relevant for development of improved interventions for disease control.

• Item Type: Articles
• Additional Information: This work was funded by the George Washington University/National Cancer Institute Cancer Biology Training Program with grant number NIH-T32CA247756 (KI) and the National Institute of Diabetes and Digestive and Kidney Diseases with grant number NIH-R01DK113504 (MHH).
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Rossi, Dr Mario and Dwomoh, Dr Louis

Creator Roles

Rossi, M.: Formal analysis, Methodology, Writing – review and editing
Dwomoh, L.: Formal analysis, Methodology, Writing – review and editing

• Authors: Osakunor, D. N.M., Ishida, K., Lamanna, O. K., Rossi, M., Dwomoh, L., and Hsieh, M. H.
• College/School: College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
• Journal Name: PLoS Neglected Tropical Diseases
• Publisher: Public Library of Science
• ISSN: 1935-2727
• ISSN (Online): 1935-2735
• Published Online: 15 February 2022
• Copyright Holders: Copyright © 2022 Osakunor et al.
• First Published: First published in PLoS Neglected Tropical Diseases 16(2): e0010176
• Publisher Policy: Reproduced under a Creative Commons License

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