Potassium channel activity in sarcolemmal vesicles formed from skeletal muscle fibres of normal and dystrophic mice

Rowe, I.C.M. , Wareham, A.C. and Whittle, M.A. (1990) Potassium channel activity in sarcolemmal vesicles formed from skeletal muscle fibres of normal and dystrophic mice. Journal of the Neurological Sciences, 98(1), pp. 51-61. (doi: 10.1016/0022-510X(90)90181-L) (PMID:2230831)

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Abstract

Sarcolemmal vesicles were produced from adult mouse extensor digitorum longus muscle (EDL) by treating swollen muscle fibres with collagenase. Vesicles formed from dystrophic (C57BL/6J dy/dy) and phenotypically normal animals were patch clamped and the single channel activity was recorded. Three types of K+ channel were observed in excised patches taken from normal and dystrophic muscle. A large conductance (300 pS) Ca2+-dependent K+ channel (KCa) was the most frequently observed of the K+ channels in both types of muscle preparation. In a number of patches taken from dystrophic muscle the open probability-voltage relationship for the KCa channel was markedly different from that in normal muscle, suggesting a possible reduction in Ca2+ sensitivity. An ATP-sensitive K+ channel (90 pS) was common to both normal and dystrophic muscle vesicles and was present in a large number of patches. An inwardly rectifying K+ channel (40 pS) was also observed in both types of sarcolemmal vesicles. The properties of all three K+ channels types were broadly consistent with other observations of skeletal muscle K+ channels, though all had higher conductances than had previously been noted in other species.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Rowe, Dr Iain
Authors: Rowe, I.C.M., Wareham, A.C., and Whittle, M.A.
College/School:College of Medical Veterinary and Life Sciences > School of Life Sciences
Journal Name:Journal of the Neurological Sciences
Publisher:Elsevier
ISSN:0022-510X
ISSN (Online):1878-5883

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