Naula, C. and Burchmore, R. (2020) 2D gel electrophoresis analysis of leishmania proteomes. In: Michels, P. A. M., Ginger, M. L. and Zilberstein, D. (eds.) Trypanosomatids: Methods and Protocols. Series: Methods in Molecular Biology. Springer US: New York, pp. 577-586. ISBN 9781071602942 (doi: 10.1007/978-1-0716-0294-2_34)
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Abstract
2D gel electrophoresis enables resolution of intact proteins in complex mixtures and is thus useful for comparative proteomic analysis, particularly of posttranslationally modified proteoforms that might not be distinguished by shotgun proteomic analysis of peptides. 2D gel electrophoresis is a multistep procedure that can require sample-specific optimization. We present a comprehensive protocol that is effective for 2D electrophoretic analysis of proteins from Leishmania promastigotes and may also be employed for Leishmania amastigotes and for trypanosomes.
| Item Type: | Book Sections |
|---|---|
| Status: | Published |
| Glasgow Author(s) Enlighten ID: | Burchmore, Dr Richard and Naula, Dr Christina |
| Authors: | Naula, C., and Burchmore, R. |
| College/School: | College of Medical Veterinary and Life Sciences College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Methods in Molecular Biology |
| Publisher: | Springer US |
| ISSN: | 1940-6029 |
| ISBN: | 9781071602942 |
| Published Online: | 28 March 2020 |
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