Miticka, H., Rowley, G., Rezuchova, B., Homerova, D., Humphreys, S., Farn, J., Roberts, M. and Kormanec, J. (2003) Transcriptional analysis of the rpoE gene encoding extracytoplasmic stress response sigma factor σE in Salmonella enteric serovar Typhimurium. FEMS Microbiology Letters, 226(2), pp. 307-314. (doi: 10.1016/S0378-1097(03)00600-1)
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Abstract
The <i>rpoE</i> gene of <i>Salmonella enterica</i> serovar Typhimurium (<i>S</i>. Typhimurium), which encodes the extracytoplasmic stress response sigma factor σ<sup>E</sup>, is critically important for the virulence of <i>S</i>. Typhimurium. We analysed expression of <i>rpoE</i> by wild-type and mutant bacteria grown in different conditions by S1-nuclease mapping using RNA, and using in vivo reporter gene fusions. Three promoters, <i>rpoEp1, rpoEp2</i> and <i>rpoEp3</i>, were located upstream of the <i>S</i>. Typhimurium <i>rpoE</i> gene. The promoters were differentially expressed during growth and under several stress conditions including cold shock. Expression from the <i>rpoEp3</i> promoter was absent in an <i>S</i>. Typhimurium <i>rpoE</i> mutant, demonstrating its dependence upon σE. The level of mRNA corresponding to <i>rpoEp3</i> was also higher in a <i>cpxR</i> mutant, indicating a negative regulation of the promoter by the Cpx system. Using this <i>rpoE</i>-dependent promoter, we optimised a two-plasmid system for identification of promoters recognised by <i>S</i>. Typhimurium σE. The <i>rpoEp3</i> promoter was active in the <i>Escherichia coli</i> two-plasmid system and has an identical transcription start point as in <i>S</i>. Typhimurium but only after induction of <i>S</i>. Typhimurium <i>rpoE</i> expression.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Roberts, Professor Mark |
Authors: | Miticka, H., Rowley, G., Rezuchova, B., Homerova, D., Humphreys, S., Farn, J., Roberts, M., and Kormanec, J. |
College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
Journal Name: | FEMS Microbiology Letters |
Publisher: | Wiley |
ISSN: | 0378-1097 |
ISSN (Online): | 1574-6968 |
Published Online: | 09 January 2006 |
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