Thermodynamic and phylogenetic prediction of RNA secondary structures in the coding region of hepatitis C virus

Tuplin, A., Wood, J., Evans, D. J., Patel, A. and Simmonds, P. (2002) Thermodynamic and phylogenetic prediction of RNA secondary structures in the coding region of hepatitis C virus. RNA, 8, pp. 824-841. (doi:10.1017/S1355838202024068)

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Abstract

We have characterized transcripts synthesized in vivo by bacteriophage T7 RNA polymerase to investigate yeast mRNA processing. T7 transcripts are not capped, consistent with capping being tightly coupled to RNA polymerase II (pol II) transcription. In contrast to higher eukaryotic non-pol II transcripts, yeast T7 transcripts are spliced as well as cleaved and polyadenylated. However, T7 and pol II transcripts are affected differently in cleavage and polyadenylation mutant strains, indicating that pol II may have a role in yeast 3′ end formation. T7 transcripts with 3′ ends directed by a polyadenylation signal are exported from the nucleus, and this export is dependent on the canonical cleavage and polyadenylation machinery. Importantly, transcripts with T7 terminator-directed 3′ ends are unadenylated and predominantly nuclear in wild-type cells. Our results suggest that transcription by pol II is required for neither the nuclear export of an in vivo-transcribed mRNA nor for the retention of transcripts with aberrant 3′ ends. Moreover, proper 3′ end formation may be necessary and sufficient to promote mRNA export in yeast.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Patel, Professor Arvind
Authors: Tuplin, A., Wood, J., Evans, D. J., Patel, A., and Simmonds, P.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:RNA
Publisher:Cold Spring Harbor Laboratory Press
ISSN:1355-8382
ISSN (Online):1469-9001

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