Flynn, J.N., Pistello, M.,, Isola, P., Zaccaro, L., Del Santo, B., Ricci, E., Matteucci, D. and Bendinelli, M. (2005) Adoptive immunotherapy of feline immunodeficiency virus with autologous ex vivo-stimulated lymphoid cells modulates virus and T-Cell subsets in blood. Clinical and Diagnostic Laboratory Immunology, 12, pp. 736-745. (doi: 10.1128/CDLI.12.6.736-745.2005)
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Abstract
The potential of immunotherapy with autologous virus-specific T cells to affect the course of feline immunodeficiency virus (FIV) infection was explored in a group of specific-pathogen-free cats infected with FIV a minimum of 10 months earlier. Popliteal lymph node cells were stimulated by cocultivation with UV-inactivated autologous fibroblasts infected with recombinant vaccinia viruses expressing either FIV gag or env gene products, followed by expansion in interleukin-2. One or two infusions of both Gag- and Env-stimulated cells resulted in a slow increase in FIV-specific gamma interferon-secreting T cells in the circulation of cats. In the same animals, viral set points fluctuated widely during the first 2 to 3 weeks after adoptive transfer and then returned to pretreatment levels. The preexisting viral quasispecies was also found to be modulated, whereas no novel viral variants were detected. Circulating CD4+ counts underwent a dramatic decline early after treatment. CD4/CD8 ratios remained instead essentially unchanged and eventually improved in some animals. In contrast, a single infusion of Gag-stimulated cells alone produced no apparent modulations of infection.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | UNSPECIFIED |
| Authors: | Flynn, J.N., Pistello, M.,, Isola, P., Zaccaro, L., Del Santo, B., Ricci, E., Matteucci, D., and Bendinelli, M. |
| College/School: | College of Medical Veterinary and Life Sciences |
| Journal Name: | Clinical and Diagnostic Laboratory Immunology |
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