Micheroli, R. et al. (2022) Role of synovial fibroblast subsets across synovial pathotypes in rheumatoid arthritis: a deconvolution analysis. RMD Open, 8(1), e001949. (doi: 10.1136/rmdopen-2021-001949) (PMID:34987094) (PMCID:PMC8734041)
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Abstract
Objectives: To integrate published single-cell RNA sequencing (scRNA-seq) data and assess the contribution of synovial fibroblast (SF) subsets to synovial pathotypes and respective clinical characteristics in treatment-naïve early arthritis. Methods: In this in silico study, we integrated scRNA-seq data from published studies with additional unpublished in-house data. Standard Seurat, Harmony and Liger workflow was performed for integration and differential gene expression analysis. We estimated single cell type proportions in bulk RNA-seq data (deconvolution) from synovial tissue from 87 treatment-naïve early arthritis patients in the Pathobiology of Early Arthritis Cohort using MuSiC. SF proportions across synovial pathotypes (fibroid, lymphoid and myeloid) and relationship of disease activity measurements across different synovial pathotypes were assessed. Results: We identified four SF clusters with respective marker genes: PRG4+ SF (CD55, MMP3, PRG4, THY1neg); CXCL12+ SF (CXCL12, CCL2, ADAMTS1, THY1low); POSTN+ SF (POSTN, collagen genes, THY1); CXCL14+ SF (CXCL14, C3, CD34, ASPN, THY1) that correspond to lining (PRG4+ SF) and sublining (CXCL12+ SF, POSTN+ + and CXCL14+ SF) SF subsets. CXCL12+ SF and POSTN+ + were most prominent in the fibroid while PRG4+ SF appeared highest in the myeloid pathotype. Corresponding, lining assessed by histology (assessed by Krenn-Score) was thicker in the myeloid, but also in the lymphoid pathotype + the fibroid pathotype. PRG4+ SF correlated positively with disease severity parameters in the fibroid, POSTN+ SF in the lymphoid pathotype whereas CXCL14+ SF showed negative association with disease severity in all pathotypes. Conclusion: This study shows a so far unexplored association between distinct synovial pathologies and SF subtypes defined by scRNA-seq. The knowledge of the diverse interplay of SF with immune cells will advance opportunities for tailored targeted treatments.
Item Type: | Articles |
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Keywords: | Rheumatoid arthritis, 1506, synovitis, fibroblasts, arthritis, rheumatoid. |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Kurowska-Stolarska, Professor Mariola and MacDonald, Miss Lucy |
Authors: | Micheroli, R., Elhai, M., Edalat, S., Frank-Bertoncelj, M., Bürki, K., Ciurea, A., MacDonald, L., Kurowska-Stolarska, M., Lewis, M. J., Goldmann, K., Cubuk, C., Kuret, T., Distler, O., Pitzalis, C., and Ospelt, C. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Research Centre: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Immunobiology |
Journal Name: | RMD Open |
Publisher: | BMJ Publishing Group |
ISSN: | 2056-5933 |
ISSN (Online): | 2056-5933 |
Copyright Holders: | Copyright © 2022 The Authors |
First Published: | First published in RMD Open 8(1):e001949 |
Publisher Policy: | Reproduced under a Creative Commons licence |
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