New members of the Escherichia coli sigma(E) regulon identified by a two-plasmid system

Rezuchova, B., Miticka, H., Homerova, D., Roberts, M. and Kormanec, J. (2003) New members of the Escherichia coli sigma(E) regulon identified by a two-plasmid system. FEMS Microbiology Letters, 225(1), pp. 1-7. (doi: 10.1016/S0378-1097(03)00480-4)

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Publisher's URL: http://dx.doi.org/10.1016/S0378-1097(03)00480-4

Abstract

A previously established method, based on a two-plasmid system, was used to identify promoters recognized by RNA polymerase containing the extracytoplasmic stress response sigma factor σ<sup>E</sup> in <i>Escherichia coli</i>. In addition to previously identified <i>rpoE</i>-dependent promoters, 11 new promoters potentially directing the expression of 15 genes were identified that were active only after over-expression of <i>rpoE</i>. The promoters were confirmed and transcriptional start points of the promoters were determined by primer extension analysis and S1-nuclease mapping. All the promoters contained sequences similar to the consensus sequence of <i>rpoE</i>-dependent promoters. The new <i>rpoE</i>-dependent promoters governed expression of genes encoding proteins involved in primary metabolism (<i>fusA, tufA, recR</i>), phospholipid and lipopolysaccharide biosynthesis (<i>psd, lpxP</i>), signal transduction (<i>sixA</i>), proposed inner or outer membrane proteins (<i>bacA, sbmA, smpA, yeaY</i>), and proteins with unknown function (<i>ybaB, yaiW, yiiS, yiiT, yfeY</i>).

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Roberts, Professor Mark
Authors: Rezuchova, B., Miticka, H., Homerova, D., Roberts, M., and Kormanec, J.
College/School:College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
Journal Name:FEMS Microbiology Letters
Publisher:Wiley
ISSN:0378-1097
ISSN (Online):1574-6968
Published Online:09 January 2006

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