Faecal proteomics in the identification of biomarkers to differentiate canine chronic enteropathies

O'Reilly, E. L., Horvatić, A., Kuleš, J., Gelemanović, A., Mrljak, V., Huang, Y., Brady, N., Chadwick, C. C., Eckersall, P. D. and Ridyard, A. (2022) Faecal proteomics in the identification of biomarkers to differentiate canine chronic enteropathies. Journal of Proteomics, 254, 104452. (doi: 10.1016/j.jprot.2021.104452) (PMID:34958965)

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Abstract

Canine chronic enteropathy (CCE) is a collective term used to describe a group of idiopathic enteropathies of dogs that result in a variety of clinical manifestations of intestinal dysfunction. Clinical stratification into food-responsive enteropathy (FRE) or non-food responsive chronic inflammatory enteropathy (CIE), is made retrospectively based on response to treatments. Faecal extracts from those with a FRE (n = 5) and those with non-food responsive chronic inflammatory enteropathies (CIE) (n = 6) were compared to a healthy control group (n = 14) by applying TMT-based quantitative proteomic approach. Many of the proteins with significant differential abundance between groups were pancreatic or intestinal enzymes with pancreatitis-associated protein (identified as REG3α) and pancreatic M14 metallocarboxypeptidase proteins carboxypeptidase A1 and B identified as being of significantly increased abundance in the CCE group. The reactome analysis revealed the recycling of bile acids and salts and their metabolism to be present in the FRE group, suggesting a possible dysbiotic aetiology. Several acute phase proteins were significantly more abundant in the CCE group with the significant increase in haptoglobin in the CIE group especially notable. Further research of these proteins is needed to fully assess their clinical utility as faecal biomarkers for differentiating CCE cases.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Horvatic, Ms Anita and Huang, Yixin and Brady, Mrs Nicola and Eckersall, Professor David and O'Reilly, Dr Emily and Ridyard, Ms Alison and Kules, Mrs Josipa
Authors: O'Reilly, E. L., Horvatić, A., Kuleš, J., Gelemanović, A., Mrljak, V., Huang, Y., Brady, N., Chadwick, C. C., Eckersall, P. D., and Ridyard, A.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:Journal of Proteomics
Publisher:Elsevier
ISSN:1874-3919
ISSN (Online):1876-7737
Published Online:24 December 2021
Copyright Holders:Copyright © 2022 Elsevier B.V.
First Published:First published in Journal of Proteomics 254:104452
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
173681Using faecal proteomics to identify biomarkers that differentiate gastrointestinal disease in dogsEmily O'ReillyPetplan Charitable Trust (PETPLAN)Project No 218/428Institute of Biodiversity, Animal Health and Comparative Medicine
163069CASE Studentship BBSRC/Aviagen 2010/11Peter EckersallBiotechnology and Biological Sciences Research Council (BBSRC)BB/H016171/1Institute of Biodiversity, Animal Health and Comparative Medicine