Label‐free cell migration assay using magnetic exclusion

Gupta, T., Gupta, R. , Dabaghi, M., Sahu, R. P., Hirota, J. A. and Puri, I. K. (2021) Label‐free cell migration assay using magnetic exclusion. Advanced Materials Technologies, (doi: 10.1002/admt.202101033) (Early Online Publication)

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Abstract

In vitro wound healing assays are widely used to investigate cell migration during various physiologic and pathologic processes. However, traditional scratch-based assays produce cell-free areas that are not reproducible, whereas the alternate insert-based exclusion method is expensive and time-consuming. Here, a rapid, label-free, insert-free magnetic exclusion technique, where magnetic fields are used to create cell-free areas is described. Suspensions of diamagnetic cells in a paramagnetic culture medium are seeded into microwells placed on an array of the coaxially arranged ring and cylinder magnets. In the presence of an inhomogeneous field, the magnetic susceptibility difference drives the cells toward an annular region of the lowest field strength. Annular cell aggregates of reproducible dimensions are produced on tissue culture-treated and collagen I-fibronectin coated surfaces within 3 h. The effects of the paramagnetic agent on cell viability, metabolism, and gene expression are investigated. A mathematical model reveals the dynamics of the cell-free area closure. The magnetic exclusion technique is inexpensive, easy to use, and amenable to automation. Potential applications include cancer research, high throughput drug discovery, and screening.

Item Type:Articles
Status:Early Online Publication
Refereed:Yes
Glasgow Author(s) Enlighten ID:Gupta, Dr Rohit
Authors: Gupta, T., Gupta, R., Dabaghi, M., Sahu, R. P., Hirota, J. A., and Puri, I. K.
College/School:College of Science and Engineering > School of Engineering > Systems Power and Energy
Journal Name:Advanced Materials Technologies
Publisher:Wiley
ISSN:2365-709X
ISSN (Online):2365-709X
Published Online:08 December 2021

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