An in vitro evaluation of denture cleansing regimens against a polymicrobial denture biofilm model

Brown, J. L. , Young, T., McKloud, E., Butcher, M. C., Bradshaw, D., Pratten, J. R. and Ramage, G. (2022) An in vitro evaluation of denture cleansing regimens against a polymicrobial denture biofilm model. Antibiotics, 11(1), 113. (doi: 10.3390/antibiotics11010113) (PMID:35052990) (PMCID:PMC8772836)

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Abstract

Denture stomatitis (DS) is an inflammatory disease resulting from a polymicrobial biofilm perturbation at the denture surface–palatal mucosa interface. Recommendations made by dental health care professionals often lack clarity for appropriate denture cleaning. This study investigated the efficacy of brushing with off-the-shelf denture cleanser (DC) tablets (Poligrip®) vs. two toothpastes (Colgate® and Crest®) in alleviating the viable microorganisms (bacteria and fungi) in an in vitro denture biofilm model. Biofilms were grown on poly(methyl)methacrylate (PMMA) discs, then treated daily for 7 days with mechanical disruption (brushing), plus Poligrip® DC, Colgate® or Crest® toothpastes. Weekly treatment with Poligrip® DC on day 7 only was compared to daily modalities. All treatment parameters were processed to determine viable colony forming units for bacteria and fungi using the Miles and Misra technique, and imaged by confocal laser scanning microscopy (CLSM). Brushing with daily DC therapy was the most effective treatment in reducing the viable biofilm over 7 days of treatment. Brushing only was ineffective in controlling the viable bioburden, which was confirmed by CLSM imaging. This data indicates that regular cleansing of PMMA with DC was best for polymicrobial biofilms.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Butcher, Mr Mark and Ramage, Professor Gordon and Brown, Dr Jason and McKloud, Emily and Young, Ms Tracy
Authors: Brown, J. L., Young, T., McKloud, E., Butcher, M. C., Bradshaw, D., Pratten, J. R., and Ramage, G.
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
Journal Name:Antibiotics
Publisher:MDPI
ISSN:2079-6382
ISSN (Online):2079-6382
Published Online:16 January 2022
Copyright Holders:Copyright © 2022 The Authors
First Published:First published in Antibiotics 11(1): 113
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
312246Using Big Data to Model the Maintenance of Health in the Human Oral CavityGordon RamageBiotechnology and Biological Sciences Research Council (BBSRC)BB/V509541/1Med - Dental School