Limited replication of human cytomegalovirus in a trophoblast cell line

Hyde, K., Sultana, N., Tran, A. C., Bileckaja, N., Donald, C. L. , Kohl, A. , Stanton, R. J. and Strang, B. L. (2021) Limited replication of human cytomegalovirus in a trophoblast cell line. Journal of General Virology, 102(11), 001683. (doi: 10.1099/jgv.0.001683)

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Abstract

Several viruses, including human cytomegalovirus (HCMV), are thought to replicate in the placenta. However, there is little understanding of the molecular mechanisms involved in HCMV replication in this tissue. We investigated replication of HCMV in the extravillous trophoblast cell line SGHPL-4, a commonly used model of HCMV replication in the placenta. We found limited HCMV protein expression and virus replication in SGHPL-4 cells. This was associated with a lack of trophoblast progenitor cell protein markers in SGHPL-4 cells, suggesting a relationship between trophoblast differentiation and limited HCMV replication. We proposed that limited HCMV replication in trophoblast cells is advantageous to vertical transmission of HCMV, as there is a greater opportunity for vertical transmission when the placenta is intact and functional. Furthermore, when we investigated the replication of other vertically transmitted viruses in SGHPL-4 cells we found some limitation to replication of Zika virus, but not herpes simplex virus. Thus, limited replication of some, but not all, vertically transmitted viruses may be a feature of trophoblast cells.

Item Type:Articles
Additional Information:This work was supported by a Wellcome Trust Institutional Strategic Support Fund (204809/Z/16/Z to B. L. S.) and the UK Medical Research Council (MC_UU_12014/8 and MR/N017552/1 to A. K., MR/S00971X/1 to R. J. S.).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Donald, Dr Claire and Kohl, Professor Alain
Creator Roles:
Donald, C. L.Methodology, Resources, Writing – review and editing
Kohl, A.Methodology, Resources, Writing – review and editing, Funding acquisition
Authors: Hyde, K., Sultana, N., Tran, A. C., Bileckaja, N., Donald, C. L., Kohl, A., Stanton, R. J., and Strang, B. L.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation > Centre for Virus Research
Journal Name:Journal of General Virology
Publisher:Microbiology Society
ISSN:0022-1317
ISSN (Online):1465-2099
Published Online:24 November 2021
Copyright Holders:Copyright © 2021 The Authors
First Published:First published in Journal of General Virology 102(11):001683
Publisher Policy:Reproduced under a Creative Commons Licence

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
172580The emergence of Zika virus in Brazil: investigating prevalence and host responses to design preventive strategiesAlain KohlMedical Research Council (MRC)MR/N017552/1III-MRC-GU Centre for Virus Research